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Literature summary for 4.2.3.B66 extracted from

  • Tezuka, D.; Ito, A.; Mitsuhashi, W.; Toyomasu, T.; Imai, R.
    The rice ent-kaurene synthase like 2 encodes a functional ent-beyerene synthase (2015), Biochem. Biophys. Res. Commun., 460, 766-771 .
    View publication on PubMed
Search for more literature for 4.2.3.B66

Cloned(Commentary)

Cloned (Comment) Organism
gene OsKSL2, full-length enzyme DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21 Oryza sativa Japonica Group

Protein Variants

Protein Variants Comment Organism
additional information construction of a recombinant gene where GFP is fused to the putative chloroplast transit peptide (cTP) sequence of OsKSL2. The fusion gene is transiently expressed in rice leaf protoplasts. GFP signals from the transformed cells overlap with autofluorescence from chloroplasts, indicating that OsKSL2 is localized to the plastid. The subcellular localization of the recombinant GFP-tagged enzyme with GFP fused to a shorter transit peptide sequence from gene Os04g0612000 (UniProt ID Q0JA81) in protoplast cells does not localize to the plastids, the shorter sequence fails to function as a transit peptide Oryza sativa Japonica Group

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast the first 58 residues of OsKSL2 function as a putative chloroplast transit peptide Oryza sativa Japonica Group 9507
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enzyme-bound Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ent-copalyl diphosphate Oryza sativa Japonica Group
-
 ent-beyerene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group A0A0F7QZZ2 cv. Yukihikari
-

Reaction

Reaction Comment Organism Reaction ID
ent-copalyl diphosphate = ent-beyerene + diphosphate reaction mechanism via carbocation intermediate, in comparison to ant-kaurene synthesis from ent-copalyl diphosphate (EC 4.2.3.19), mass spectrometric analysis Oryza sativa Japonica Group
a

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Oryza sativa Japonica Group ent-beyerene + diphosphate
-
 ?
additional information recombinant OsKSL2 enzyme catalyzes the cyclization of ent-copalyl diphosphate into ent-beyerene as a major and ent-kaurene as a minor product, cf. EC 4.2.3.19, GC-MS product analysis Oryza sativa Japonica Group ?
-
 ?

Synonyms

Synonyms Comment Organism
ent-kaurene synthase like 2
-
 Oryza sativa Japonica Group
OsKSL2
-
 Oryza sativa Japonica Group

Expression

Organism Comment Expression
Oryza sativa Japonica Group OsKSL2 is induced by methyl jasmonate up

General Information

General Information Comment Organism
evolution the enzyme belongs to the class I diterpene synthases Oryza sativa Japonica Group
physiological function OsKSL2 is a functional ent-beyerene synthase that is involved in defense mechanisms in rice roots. ent-Beyerene is an antimicrobial compound Oryza sativa Japonica Group