Literature summary for 4.2.3.B32 extracted from

Hoshino, T.; Nakano, C.; Ootsuka, T.; Shinohara, Y.; Hara, T.
Substrate specificity of Rv3378c, an enzyme from Mycobacterium tuberculosis, and the inhibitory activity of the bicyclic diterpenoids against macrophage phagocytosis (2011), Org. Biomol. Chem., 9, 2156-2165.

Search for more literature for 4.2.3.B32

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essentially required	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WJ61	putative	-
Mycobacterium tuberculosis H37Rv	P9WJ61	putative	-

Reaction

Reaction	Comment	Organism	Reaction ID
(+)-copalyl diphosphate + H2O = 13-epi-manool + diphosphate	products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool. Enzyme catalyzes the removal reaction of the diphosphate moiety, followed by addition of water to the generated cation	Mycobacterium tuberculosis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis	13-epi-manool + diphosphate	products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool	?
(+)-copalyl diphosphate + H2O	-	Mycobacterium tuberculosis H37Rv	13-epi-manool + diphosphate	products of Mycobacterium tuberculosis gene product Rv3378c are 40% (+)-copalool, 12% manool, 15% 13-epi-manool	?

Synonyms

Synonyms	Comment	Organism
Rv3378c	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
45	-	-	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	9	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)