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Literature summary for 4.2.3.B31 extracted from

  • Caniard, A.; Zerbe, P.; Legrand, S.; Cohade, A.; Valot, N.; Magnard, J.L.; Bohlmann, J.; Legendre, L.
    Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture (2012), BMC Plant Biol., 12, 119.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.B31

Application

Application Comment Organism
synthesis synthesis of sclareol by co-expression of producing labda-13-en-8-ol diphosphate as major product and manool synthase in Saccharomyces cerevisiae Salvia sclarea

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and Saccharomyces cerevisiae Salvia sclarea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Salvia sclarea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Salvia sclarea

Organism

Organism UniProt Comment Textmining
Salvia sclarea K4HYB0 c.f. EC 4.2.3.141
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9S,10S)-copalyl diphosphate + H2O
-
 Salvia sclarea manool + diphosphate
-
 ?
labda-13-en-8-ol diphosphate + H2O
-
 Salvia sclarea sclareol + diphosphate
-
 ?
additional information no substrate: geranylgeranyl diphosphate Salvia sclarea ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Salvia sclarea