BRENDA - Enzyme Database
show all sequences of 4.2.3.B18

Structural elucidation of cisoid and transoid cyclization pathways of a sesquiterpene synthase using 2-fluorofarnesyl diphosphates

Noel, J.P.; Dellas, N.; Faraldos, J.A.; Zhao, M.; Hess, B.A.; Smentek, L.; Coates, R.M.; OMaille, P.E.; ACS Chem. Biol. 5, 377-392 (2010)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization (Commentary)
Organism
hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00797
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
0.01403
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2Z,6E)-farnesyl diphosphate
Nicotiana tabacum
-
(-)-alpha-cedrene + diphosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Nicotiana tabacum
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(2Z,6E)-farnesyl diphosphate
-
701441
Nicotiana tabacum
(-)-alpha-cedrene + diphosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.077
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
0.095
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00797
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
0.01403
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(2Z,6E)-farnesyl diphosphate
Nicotiana tabacum
-
(-)-alpha-cedrene + diphosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(2Z,6E)-farnesyl diphosphate
-
701441
Nicotiana tabacum
(-)-alpha-cedrene + diphosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.077
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
0.095
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.77
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
9.66
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6.77
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, wild-type enzyme
Nicotiana tabacum
9.66
-
(2Z,6E)-farnesyl diphosphate
pH 7.5, 22°C, promiscuous mutant enzyme M4
Nicotiana tabacum
Other publictions for EC 4.2.3.B18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
701441
Noel
Structural elucidation of ciso ...
Nicotiana tabacum
ACS Chem. Biol.
5
377-392
2010
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704204
Faraldos
Bisabolyl-derived sesquiterpen ...
Nicotiana tabacum
J. Am. Chem. Soc.
132
4281-4289
2010
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