Literature summary for 4.2.3.B17 extracted from

Noel, J.P.; Dellas, N.; Faraldos, J.A.; Zhao, M.; Hess, B.A.; Smentek, L.; Coates, R.M.; OMaille, P.E.
Structural elucidation of cisoid and transoid cyclization pathways of a sesquiterpene synthase using 2-fluorofarnesyl diphosphates (2010), ACS Chem. Biol., 5, 377-392.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization	Nicotiana tabacum

Crystallization (Comment)

Organism

hanging drop method, crystallographic analyses of wild-type enzyme and a promiscuous mutant, with unreactive 2-fluoro analogues ((2E,6E)-2-fluorofarnesyl diphosphate and (2Z,6E)-2-fluorofarnesyl diphosphate) of (2E,6E)- and (2Z,6E)-farnesyl diphosphate reveals catalytically relevant binding modes and distinct farnesyl chain topologies that are consistent with preorganization by the active-site for cisoid or transoid cyclization

Nicotiana tabacum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00797	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, promiscuous mutant enzyme M4	Nicotiana tabacum
0.01403	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, wild-type enzyme	Nicotiana tabacum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate	Nicotiana tabacum	-	(+)-2-epi-prezizaene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2Z,6E)-farnesyl diphosphate	-	Nicotiana tabacum	(+)-2-epi-prezizaene + diphosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.077	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, promiscuous mutant enzyme M4	Nicotiana tabacum
0.095	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, wild-type enzyme	Nicotiana tabacum

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.77	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, wild-type enzyme	Nicotiana tabacum
9.66	-	(2Z,6E)-farnesyl diphosphate	pH 7.5, 22°C, promiscuous mutant enzyme M4	Nicotiana tabacum