Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.96 extracted from

  • Chou, W.K.; Fanizza, I.; Uchiyama, T.; Komatsu, M.; Ikeda, H.; Cane, D.E.
    Genome mining in Streptomyces avermitilis: cloning and characterization of SAV 76, the synthase for a new sesquiterpene, avermitilol (2010), J. Am. Chem. Soc., 132, 8850-8851.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.96

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces avermitilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00106
-
 (2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Streptomyces avermitilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Streptomyces avermitilis

Organism

Organism UniProt Comment Textmining
Streptomyces avermitilis
-
-
-
Streptomyces avermitilis SUKA17
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Streptomyces avermitilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces avermitilis avermitilol + diphosphate avermitilol is the major product (85%), accompanied by germacrene A (1%), germacrene B (5%) and viridiflorol (3%), as well as several unidentified minor sesquiterpene products ?
(2E,6E)-farnesyl diphosphate + H2O
-
 Streptomyces avermitilis SUKA17 avermitilol + diphosphate avermitilol is the major product (85%), accompanied by germacrene A (1%), germacrene B (5%) and viridiflorol (3%), as well as several unidentified minor sesquiterpene products ?

Synonyms

Synonyms Comment Organism
SAV 76
-
 Streptomyces avermitilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
 (2E,6E)-farnesyl diphosphate pH and temperature not specified in the publication Streptomyces avermitilis