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Literature summary for 4.2.3.95 extracted from

  • Lopez-Gallego, F.; Wawrzyn, G.; Schmidt-Dannert, C.
    Selectivity of fungal sesquiterpene synthases: Role of the active sites H-1alpha loop in catalysis (2010), Appl. Environ. Microbiol., 76, 7723-7733.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.95

Protein Variants

Protein Variants Comment Organism
C236A the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% sesquiterpene products from (2E,6E)-farnesyl diphosphate (41.2% of wild type activity) Coprinopsis cinerea
E237L the mutant produces 96% (-)-alpha-cuprenene, 3% gamma-cuprenene and 1% unidentified sesquiterpene products from (2E,6E)-farnesyl diphosphate (59.4% of wild type activity) Coprinopsis cinerea
additional information in the Cop6 loop graft mutant Cop6L4, loop replacement has no influence on the selectivity of the enzyme for alpha-cuprenene, the mutation reduces the catalytic efficiency 3-17fold Coprinopsis cinerea
N240L the mutant produces 98% (-)-alpha-cuprenene and 2% gamma-cuprenene from (2E,6E)-farnesyl diphosphate (31% of wild type activity) Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
0.0125
-
 (2E,6E)-farnesyl diphosphate mutant enzyme C236A, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
0.0136
-
 (2E,6E)-farnesyl diphosphate mutant enzyme E237L, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
0.04
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N240L, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Coprinopsis cinerea (-)-alpha-cuprenene + diphosphate major product is (-)-alpha-cuprenene (98% yield) minor product is gamma-cuprenene (2% yield) ?

Synonyms

Synonyms Comment Organism
(-)-alpha-cuprenene synthase
-
 Coprinopsis cinerea
Cop6
-
 Coprinopsis cinerea

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.246
-
 (2E,6E)-farnesyl diphosphate mutant enzyme N240L, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
21.3
-
 (2E,6E)-farnesyl diphosphate mutant enzyme C236A, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
28.3
-
 (2E,6E)-farnesyl diphosphate mutant enzyme E237L, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea
88
-
 (2E,6E)-farnesyl diphosphate wild type enzyme, in 50 mM Tris-HCl, 10 mM MgCl2 (pH 8.0), at 30°C Coprinopsis cinerea