Literature summary for 4.2.3.91 extracted from

Lopez-Gallego, F.; Wawrzyn, G.; Schmidt-Dannert, C.
Selectivity of fungal sesquiterpene synthases: Role of the active sites H-1alpha loop in catalysis (2010), Appl. Environ. Microbiol., 76, 7723-7733.

Search for more literature for 4.2.3.91

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain JM109 or BL21	Coprinopsis cinerea

Protein Variants

Protein Variants	Comment	Organism
4L6	the mutant shows a 1000fold-decreased catalytic efficiency compared to the wild type enzyme	Coprinopsis cinerea
H235P	the mutant makes (-)-germacrene D as the major cyclization product and also synthesizes beta-ylangene not synthesized by wild-type Cop4	Coprinopsis cinerea
K233I	the mutation has only a moderate effect on product selectivity	Coprinopsis cinerea
N239L	the mutant makes (-)-germacrene D as the major cyclization product and also synthesizes beta-ylangene not synthesized by wild-type Cop4	Coprinopsis cinerea

Organism

Organism	UniProt	Comment	Textmining
Coprinopsis cinerea	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate + H2O	-	Coprinopsis cinerea	cubebol + diphosphate	germacrene D and cubebol are the major products in vitro	?
additional information	Cop4 rearranges a secondary cisoid, allylic cation derived from farnesyl diphophate after isomerization (cis-trans pathway of catalysis) into multiple products, with delta-cadinene and beta-copaene as the major products when expressed in Escherichia coli	Coprinopsis cinerea	?	-	?

Synonyms

Synonyms	Comment	Organism
Cop4	-	Coprinopsis cinerea

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Release 2023.1 (January 2023)