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Literature summary for 4.2.3.90 extracted from

  • Agger, S.A.; Lopez-Gallego, F.; Hoye, T.R.; Schmidt-Dannert, C.
    Identification of sesquiterpene synthases from Nostoc punctiforme PCC 73102 and Nostoc sp. strain PCC 7120 (2008), J. Bacteriol., 190, 6084-6096.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.90

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Nostoc sp.
expressed in Escherichia coli BL21(DE3) cells Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
 (E,E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc punctiforme
0.0034
-
 (2E,6E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc sp.

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
-
-
-
Nostoc sp.
-
-
-
Nostoc sp. PCC 712
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon metal affinity column chromatography Nostoc sp.
Talon metal affinity column chromatography Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Nostoc sp. 8a-epi-alpha-selinene + diphosphate
-
 ?
(2E,6E)-farnesyl diphosphate
-
 Nostoc sp. PCC 712 8a-epi-alpha-selinene + diphosphate
-
 ?
(E,E)-farnesyl diphosphate
-
 Nostoc punctiforme 8a-epi-alpha-selinene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
NP1
-
 Nostoc punctiforme
NS1
-
 Nostoc sp.
terpene synthase
-
 Nostoc sp.
terpene synthase
-
 Nostoc punctiforme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.044
-
 (2E,6E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc sp.
0.063
-
 (E,E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc punctiforme

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13000
-
 (2E,6E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc sp.
24000
-
 (E,E)-farnesyl diphosphate in 10 mM Tris-HCl, 10 mM MgCl2, 1 mM beta-mercaptoethanol, pH 8.0, at 30°C Nostoc punctiforme