Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzymes in complex with (2E,6E)-farnesyl diphosphate or 4, sitting drop vapor diffusion method, using 20% PEG 3350 as precipitant | Aspergillus terreus |
Protein Variants | Comment | Organism |
---|---|---|
N299A | the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions of 50% germacrene A and 50% aristolochene | Aspergillus terreus |
N299A/S303A | the decreasing have modest effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E)-nerolidol and aristolochene as by-products | Aspergillus terreus |
N299L | the mutation has decreasing effects on sesquiterpene product distributions with germacrene A and aristolochene as main product and (E)-nerolidol as by-product | Aspergillus terreus |
N299V | inactive | Aspergillus terreus |
Q151E | the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with aristolochene and germacrene A as main products, and (E)-nerolidol and (E,E)-farnesol as by-products | Aspergillus terreus |
Q151H | the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products | Aspergillus terreus |
S303A | the mutation has increasing effects on catalysis and significant effects on sesquiterpene product distributions with germacrene A and (E)-nerolidol as by-products | Aspergillus terreus |
S303D | the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A and (E,E)-farnesol as main products and (E)-nerolidol and aristolochene as by-products | Aspergillus terreus |
S303H | the mutation has decreasing effects on catalysis but significant effects on sesquiterpene product distributions with germacrene A as main product and (E,E)-farnesol, (E)-nerolidol and aristolochene as by-products | Aspergillus terreus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000042 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151H, at pH 8.0 and 30°C | Aspergillus terreus | |
0.000061 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303H, at pH 8.0 and 30°C | Aspergillus terreus | |
0.000099 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, at pH 8.0 and 30°C | Aspergillus terreus | |
0.0001 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151E, at pH 8.0 and 30°C | Aspergillus terreus | |
0.00012 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303A, at pH 8.0 and 30°C | Aspergillus terreus | |
0.00036 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303D, at pH 8.0 and 30°C | Aspergillus terreus | |
0.00041 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A, at pH 8.0 and 30°C | Aspergillus terreus | |
0.00041 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A/S303A, at pH 8.0 and 30°C | Aspergillus terreus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aspergillus terreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Aspergillus terreus | - |
aristolochene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus terreus | Q9UR08 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration | Aspergillus terreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Aspergillus terreus | aristolochene + diphosphate | - |
? | |
additional information | no activity with farnesyl S-thiolodiphosphate | Aspergillus terreus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATAS | - |
Aspergillus terreus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00041 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303D, at pH 8.0 and 30°C | Aspergillus terreus | |
0.00063 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151H, at pH 8.0 and 30°C | Aspergillus terreus | |
0.0013 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303H, at pH 8.0 and 30°C | Aspergillus terreus | |
0.0015 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151E, at pH 8.0 and 30°C | Aspergillus terreus | |
0.0058 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A/S303A, at pH 8.0 and 30°C | Aspergillus terreus | |
0.012 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A, at pH 8.0 and 30°C | Aspergillus terreus | |
0.027 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, at pH 8.0 and 30°C | Aspergillus terreus | |
0.045 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303A, at pH 8.0 and 30°C | Aspergillus terreus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303D, at pH 8.0 and 30°C | Aspergillus terreus | |
14 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A/S303A, at pH 8.0 and 30°C | Aspergillus terreus | |
15 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151E, at pH 8.0 and 30°C | Aspergillus terreus | |
15 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme Q151H, at pH 8.0 and 30°C | Aspergillus terreus | |
21 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303H, at pH 8.0 and 30°C | Aspergillus terreus | |
29 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme N299A, at pH 8.0 and 30°C | Aspergillus terreus | |
270 | - |
(2E,6E)-farnesyl diphosphate | wild type enzyme, at pH 8.0 and 30°C | Aspergillus terreus | |
370 | - |
(2E,6E)-farnesyl diphosphate | mutant enzyme S303A, at pH 8.0 and 30°C | Aspergillus terreus |