Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Nicotiana tabacum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
anilinogeranyl diphosphate | potent noncompetitive inhibitor | Nicotiana tabacum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00447 | - |
(2E,6E)-farnesyl diphosphate | at pH 7.5 and 37°C | Nicotiana tabacum | |
0.0082 | - |
anilinogeranyl diphosphate | at pH 7.5 and 37°C | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Nicotiana tabacum | - |
(+)-5-epi-aristolochene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | Q40577 | - |
- |
Purification (Comment) | Organism |
---|---|
His-bind Ni2+ column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Nicotiana tabacum | (+)-5-epi-aristolochene + diphosphate | - |
? | |
anilinogeranyl diphosphate | - |
Nicotiana tabacum | (4E,8E)-4,8-dimethyl-2-azabicyclo[9.2.2]pentadeca-1(13),4,8,11,14-pentaene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-epi-aristolochene synthase | - |
Nicotiana tabacum |
EAS | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00012 | - |
anilinogeranyl diphosphate | at pH 7.5 and 37°C | Nicotiana tabacum | |
0.032 | - |
(2E,6E)-farnesyl diphosphate | at pH 7.5 and 37°C | Nicotiana tabacum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00041 | - |
anilinogeranyl diphosphate | at pH 7.5 and 37°C | Nicotiana tabacum |