KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0143 | - |
(2Z,6E)-farnesyl diphosphate | in 200 mM Tris-HCl (pH 7.5), 40 mM MgCl2, at 22°C | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Nicotiana tabacum | (+)-5-epiaristolochene + diphosphate | in addition to the major products (+)-5-epiaristolochene (78.9%), its DELTA1(10) isomer (-)-4-epieremophilene (6.2%), and (R)-germacrene A (3.7%), incubations of (2Z,6E)-farnesyl diphosphate with the enzyme lead to 22 additional sesquiterpenes (11% of total products), among the identified minor products are (-)-R-cedrene, an isomer of (-)-prezizaene and an acoradiene (together accounting for 2.5% of the total hydrocarbon fraction) | ? | |
(2Z,6E)-farnesyl diphosphate | - |
Nicotiana tabacum | (+)-2-epiprezizaene + (-)-alpha-cedrene + (-)-beta-curcumene + alpha-acoradiene + 4-epi-alpha-acoradiene + alpha-bisabolol + epi-alpha-bisabolol + nerolidol + (2Z,6E)-farnesol + diphosphate | (+)-2-epiprezizaene (44% yield), (-)-alpha-cedrene (21.5% yield), (-)-beta-curcumene (15.5% yield), alpha-acoradiene (3.9% yield), 4-epi-alpha-acoradiene (1.3% yield), alpha-bisabolol (1.8% yield), epi-alpha-bisabolol (1.8% yield), nerolidol (3.6% yield), (2Z,6E)-farnesol (6.7% yield) | ? |
Synonyms | Comment | Organism |
---|---|---|
5-epi-aristolochene synthase | - |
Nicotiana tabacum |
TEAS | - |
Nicotiana tabacum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.095 | - |
(2Z,6E)-farnesyl diphosphate | in 200 mM Tris-HCl (pH 7.5), 40 mM MgCl2, at 22°C | Nicotiana tabacum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0068 | - |
(2Z,6E)-farnesyl diphosphate | in 200 mM Tris-HCl (pH 7.5), 40 mM MgCl2, at 22°C | Nicotiana tabacum | |
5 | - |
(2E,6E)-farnesyl diphosphate | - |
Nicotiana tabacum |