Literature summary for 4.2.3.89 extracted from
Yang, J.; Li, Z.; Guo, L.; Du, J.; Bae, H.
Biosynthesis of beta-caryophyllene, a novel terpene-based high-density biofuel precursor, using engineered Escherichia coli (2016), Renewable Energy, 99, 216-223 .
No PubMed abstract available
Application
Application |
Comment |
Organism |
biofuel production |
acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce b-caryophyllene from reproducible sources. Contribution by recombinant production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain |
Zea perennis |
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene tps23, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH |
Zea perennis |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1-deoxy-D-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways are employed. Geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene), and beta-caryophyllene synthase genes are co-overexpressed in the engineered strain. The final genetically modified strain, YJM59, produces 220 mg/l of beta-caryophyllene in flask culture. Evaluation of fed-batch fermentation for the production of beta-caryophyllene. After induction for 60 h, the YJM59 strain produces beta-caryophyllene at a concentration of 1520 mg/l. The volumetric production fermented in the aerobic fed-batch is 0.34 mg/(l/h/OD600) and the conversion efficiency of glucose to beta-caryophyllene (gram to gram) is 1.69%. Method evaluation with beta-caryophyllene synthases from different origins, QHS1 from Artemisia annua is the most effective of the three enzymes, compared to TPS21 from Arabidopsis thaliana and TPS23 from Zea perennis. Substrate channeling |
Zea perennis |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
(2E,6E)-farnesyl diphosphate |
Zea perennis |
- |
(+)-beta-caryophyllene + diphosphate |
- |
? |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Zea perennis |
B2C4D8 |
i.e Euchlaena perennis |
- |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
(2E,6E)-farnesyl diphosphate |
- |
Zea perennis |
(+)-beta-caryophyllene + diphosphate |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
tps23 |
- |
Zea perennis |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
30 |
- |
in vivo assay at |
Zea perennis |
General Information
General Information |
Comment |
Organism |
metabolism |
beta-caryophyllene is produced via the mevalonate (MVA)-mediated pathway, overview |
Zea perennis |