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Literature summary for 4.2.3.89 extracted from

  • Li, F.; Fu, N.; Zhou, J.; Wang, G.
    Functional characterization of (E)-beta-caryophyllene synthase from lima bean and its up-regulation by spider mites and alamethicin (2017), J. Integr. Agric., 16, 2231-2238 .
No PubMed abstract available
Search for more literature for 4.2.3.89

Cloned(Commentary)

Cloned (Comment) Organism
gene PlCAHS, DNA and amino acid sequence determination and analysis,sequence comparisons and phylogenetic analysis, real time quantitative PCR expression analysis, recombinant expression in Escherichia coli strain BL21(DE3) Phaseolus lunatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Phaseolus lunatus
-
 (+)-beta-caryophyllene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Phaseolus lunatus A0A2K8GLY5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Phaseolus lunatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Phaseolus lunatus (+)-beta-caryophyllene + diphosphate
-
 ?
additional information the recombinant enzyme catalyses the formation of a major product (E)-beta-caryophyllene (82%) and a minor product alpha-humulene (18%) from farnesyl diphosphate Phaseolus lunatus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 67950, sequence calculation Phaseolus lunatus

Synonyms

Synonyms Comment Organism
(E)-beta-caryophyllene synthase
-
 Phaseolus lunatus
PlCAHS
-
 Phaseolus lunatus

Expression

Organism Comment Expression
Phaseolus lunatus enzyme PlCAHS transcript is significantly upregulated in leaves after treatment with spider mites and alamethicin (ALA) suggesting its ecological function in plant defence up

General Information

General Information Comment Organism
evolution the enzyme belongs to the terpene synthase family, TPS-a subfamily Phaseolus lunatus
physiological function (E)-beta-caryophyllene synthase is a possible key enzyme of plant defence Phaseolus lunatus