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Literature summary for 4.2.3.89 extracted from

  • Yahyaa, M.; Tholl, D.; Cormier, G.; Jensen, R.; Simon, P.W.; Ibdah, M.
    Identification and characterization of terpene synthases potentially involved in the formation of volatile terpenes in carrot (Daucus carota L.) Roots (2015), J. Agric. Food Chem., 63, 4870-4878 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene DcTPS1, cloned from wild Daucus carrota carota fruits (accession 23727), unrooted neighbor-joining phylogenetic tree, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Daucus carota subsp. carota

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Daucus carota subsp. carota
Mn2+ required Daucus carota subsp. carota

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Daucus carota subsp. carota
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(+)-beta-caryophyllene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Daucus carota subsp. carota
-
from North Golan
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Daucus carota subsp. carota

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Daucus carota subsp. carota
-
fruit
-
Daucus carota subsp. carota
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leaf
-
Daucus carota subsp. carota
-
additional information tissue expression analysis of enzyme TPS1. The highest expression of WtDcTPS1 gene occurs in the fruits, whereas the lowest transcript levels are observed in the flowers, fruit pericarp, and roots. The expression level of the WtDcTPS1 gene in the fruits of the wild type carrot accession 23727 is 40fold higher than that found in the roots, leaves, flowers, and pericarp Daucus carota subsp. carota
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pericarp
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Daucus carota subsp. carota
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root
-
Daucus carota subsp. carota
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Daucus carota subsp. carota (+)-beta-caryophyllene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
DcTPS1
-
Daucus carota subsp. carota
TPS1
-
Daucus carota subsp. carota
WtDcTPS
-
Daucus carota subsp. carota

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Daucus carota subsp. carota

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Daucus carota subsp. carota

General Information

General Information Comment Organism
evolution gene WtDcTPS1 exhibits highly conserved sequences of plant TPSs including the asparate-rich motif DDxxD and the RxR motif, both of which are involved in catalysis Daucus carota subsp. carota
additional information monoterpene contents comparisons of different wild accessions of carrot. In cell-free extracts from carrot fruit pericarp of wild accession 23727, the monoterpenes contained are geraniol, sabinene, limonene, alpha-pinene, beta-pinene, beta-myrcene, and alpha-phellandrene and the sesquiterpenes are E-beta-caryophellene, E-beta-farnesene, and alpha-humelene Daucus carota subsp. carota