Cloned (Comment) | Organism |
---|---|
gene DcTPS1, cloned from wild Daucus carrota carota fruits (accession 23727), unrooted neighbor-joining phylogenetic tree, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Daucus carota subsp. carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Daucus carota subsp. carota | |
Mn2+ | required | Daucus carota subsp. carota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Daucus carota subsp. carota | - |
(+)-beta-caryophyllene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota subsp. carota | - |
from North Golan | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Daucus carota subsp. carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Daucus carota subsp. carota | - |
fruit | - |
Daucus carota subsp. carota | - |
leaf | - |
Daucus carota subsp. carota | - |
additional information | tissue expression analysis of enzyme TPS1. The highest expression of WtDcTPS1 gene occurs in the fruits, whereas the lowest transcript levels are observed in the flowers, fruit pericarp, and roots. The expression level of the WtDcTPS1 gene in the fruits of the wild type carrot accession 23727 is 40fold higher than that found in the roots, leaves, flowers, and pericarp | Daucus carota subsp. carota | - |
pericarp | - |
Daucus carota subsp. carota | - |
root | - |
Daucus carota subsp. carota | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Daucus carota subsp. carota | (+)-beta-caryophyllene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DcTPS1 | - |
Daucus carota subsp. carota |
TPS1 | - |
Daucus carota subsp. carota |
WtDcTPS | - |
Daucus carota subsp. carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Daucus carota subsp. carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Daucus carota subsp. carota |
General Information | Comment | Organism |
---|---|---|
evolution | gene WtDcTPS1 exhibits highly conserved sequences of plant TPSs including the asparate-rich motif DDxxD and the RxR motif, both of which are involved in catalysis | Daucus carota subsp. carota |
additional information | monoterpene contents comparisons of different wild accessions of carrot. In cell-free extracts from carrot fruit pericarp of wild accession 23727, the monoterpenes contained are geraniol, sabinene, limonene, alpha-pinene, beta-pinene, beta-myrcene, and alpha-phellandrene and the sesquiterpenes are E-beta-caryophellene, E-beta-farnesene, and alpha-humelene | Daucus carota subsp. carota |