Cloned (Comment) | Organism |
---|---|
gene TPS1, sequence comparisons and phylogenetic tree, isozyme TPS1 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) | Rhodomyrtus tomentosa |
gene TPS3, sequence comparisons and phylogenetic tree, isozyme TPS3 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) | Rhodomyrtus tomentosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme RtTPS1 contains no typical N-terminal transit peptide (62-64aa) | Rhodomyrtus tomentosa | 5829 | - |
cytosol | isozyme RtTPS3 contains no typical N-terminal transit peptide (62-64aa) | Rhodomyrtus tomentosa | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Rhodomyrtus tomentosa | - |
beta-caryophyllene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodomyrtus tomentosa | A0A385J6X8 | - |
- |
Rhodomyrtus tomentosa | A0A385J6Y9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodomyrtus tomentosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | higher beta-caryophyllene contents in mature leaves compared to young leaves | Rhodomyrtus tomentosa | - |
additional information | isozyme expression patterns, overview | Rhodomyrtus tomentosa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Rhodomyrtus tomentosa | beta-caryophyllene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 4.2.3.57 | Rhodomyrtus tomentosa |
RtTPS1 | - |
Rhodomyrtus tomentosa |
RtTPS3 | - |
Rhodomyrtus tomentosa |
TPS1 | - |
Rhodomyrtus tomentosa |
TPS3 | - |
Rhodomyrtus tomentosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodomyrtus tomentosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhodomyrtus tomentosa |
General Information | Comment | Organism |
---|---|---|
evolution | isozyme RtTPS1 contains all the conserved domains of the TPS family, including the RR(P)X8W, RXR, and DDXXD (X is any amino acid) motifs, and absolutely conserved arginine, cysteine and histidine residues in active-site | Rhodomyrtus tomentosa |
evolution | isozyme RtTPS3 contains all the conserved domains of the TPS family, including the RR(P)X8W, RXR, and DDXXD (X is any amino acid) motifs, and absolutely conserved arginine, cysteine and histidine residues in active-site | Rhodomyrtus tomentosa |
metabolism | putative alpha-pinene and beta-caryophyllene biosynthesis pathway | Rhodomyrtus tomentosa |
additional information | isozymes RtTPS1-4 mainly produce (+)-alpha-pinene and (+)-beta-pinene, as well as small amounts of (-)-alpha-pinene and (-)-beta-pinene with geranyl diphosphate, while RtTPS1 and RtTPS3 are also active with farnesyl diphosphate, producing beta-caryophyllene (65.71% for RtTPS1 and 93.05% for RtTPS3), along with a smaller amount of alpha-humulene (4.29% for RtTPS1 and 6.95% for RtTPS3). alpha/beta-Pinene, beta-caryophyllene, and alpha-humulene constitute the major active components in Rhodomyrtus tomentosa. Among them, (+)-alpha-pinene and beta-caryophyllene are most abundant in the leaves | Rhodomyrtus tomentosa |
additional information | isozymes RtTPS1-4 mainly produce (+)-alpha-pinene and (+)-beta-pinene, as well as small amounts of (-)-alpha-pinene and (-)-beta-pinene with GPP, while RtTPS1 and RtTPS3 are also active with FPP, producing beta-caryophyllene (65.71% for RtTPS1 and 93.05% for RtTPS3), along with a smaller amount of alpha-humulene (4.29% for RtTPS1 and 6.95% for RtTPS3). alpha/beta-Pinene, beta-caryophyllene, and alpha-humulene constitute the major active components in Rhodomyrtus tomentosa. Among them, (+)-alpha-pinene and beta-caryophyllene are most abundant in the leaves | Rhodomyrtus tomentosa |