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Literature summary for 4.2.3.89 extracted from

  • He, S.M.; Wang, X.; Yang, S.C.; Dong, Y.; Zhao, Q.M.; Yang, J.L.; Cong, K.; Zhang, J.J.; Zhang, G.H.; Wang, Y.; Fan, W.
    De novo transcriptome characterization of Rhodomyrtus tomentosa leaves and identification of genes involved in alpha/beta-pinene and beta-caryophyllene biosynthesis (2018), Front. Plant Sci., 9, 1231 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS1, sequence comparisons and phylogenetic tree, isozyme TPS1 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) Rhodomyrtus tomentosa
gene TPS3, sequence comparisons and phylogenetic tree, isozyme TPS3 RT-PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21(DE3) Rhodomyrtus tomentosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme RtTPS1 contains no typical N-terminal transit peptide (62-64aa) Rhodomyrtus tomentosa 5829
-
cytosol isozyme RtTPS3 contains no typical N-terminal transit peptide (62-64aa) Rhodomyrtus tomentosa 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Rhodomyrtus tomentosa
-
beta-caryophyllene + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodomyrtus tomentosa A0A385J6X8
-
-
Rhodomyrtus tomentosa A0A385J6Y9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Rhodomyrtus tomentosa

Source Tissue

Source Tissue Comment Organism Textmining
leaf higher beta-caryophyllene contents in mature leaves compared to young leaves Rhodomyrtus tomentosa
-
additional information isozyme expression patterns, overview Rhodomyrtus tomentosa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
Rhodomyrtus tomentosa beta-caryophyllene + diphosphate
-
?

Synonyms

Synonyms Comment Organism
More cf. EC 4.2.3.57 Rhodomyrtus tomentosa
RtTPS1
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Rhodomyrtus tomentosa
RtTPS3
-
Rhodomyrtus tomentosa
TPS1
-
Rhodomyrtus tomentosa
TPS3
-
Rhodomyrtus tomentosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodomyrtus tomentosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Rhodomyrtus tomentosa

General Information

General Information Comment Organism
evolution isozyme RtTPS1 contains all the conserved domains of the TPS family, including the RR(P)X8W, RXR, and DDXXD (X is any amino acid) motifs, and absolutely conserved arginine, cysteine and histidine residues in active-site Rhodomyrtus tomentosa
evolution isozyme RtTPS3 contains all the conserved domains of the TPS family, including the RR(P)X8W, RXR, and DDXXD (X is any amino acid) motifs, and absolutely conserved arginine, cysteine and histidine residues in active-site Rhodomyrtus tomentosa
metabolism putative alpha-pinene and beta-caryophyllene biosynthesis pathway Rhodomyrtus tomentosa
additional information isozymes RtTPS1-4 mainly produce (+)-alpha-pinene and (+)-beta-pinene, as well as small amounts of (-)-alpha-pinene and (-)-beta-pinene with geranyl diphosphate, while RtTPS1 and RtTPS3 are also active with farnesyl diphosphate, producing beta-caryophyllene (65.71% for RtTPS1 and 93.05% for RtTPS3), along with a smaller amount of alpha-humulene (4.29% for RtTPS1 and 6.95% for RtTPS3). alpha/beta-Pinene, beta-caryophyllene, and alpha-humulene constitute the major active components in Rhodomyrtus tomentosa. Among them, (+)-alpha-pinene and beta-caryophyllene are most abundant in the leaves Rhodomyrtus tomentosa
additional information isozymes RtTPS1-4 mainly produce (+)-alpha-pinene and (+)-beta-pinene, as well as small amounts of (-)-alpha-pinene and (-)-beta-pinene with GPP, while RtTPS1 and RtTPS3 are also active with FPP, producing beta-caryophyllene (65.71% for RtTPS1 and 93.05% for RtTPS3), along with a smaller amount of alpha-humulene (4.29% for RtTPS1 and 6.95% for RtTPS3). alpha/beta-Pinene, beta-caryophyllene, and alpha-humulene constitute the major active components in Rhodomyrtus tomentosa. Among them, (+)-alpha-pinene and beta-caryophyllene are most abundant in the leaves Rhodomyrtus tomentosa