Any feedback?
Literature summary for 4.2.3.89 extracted from
- Genomic analysis of terpene synthase family and functional characterization of seven sesquiterpene synthases from Citrus sinensis (2017), Front. Plant Sci., 8, 1481 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
| gene CsSesquiTPS6b, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
| gene CsSesquiTPS6c, DNA and amino acid sequence determination and analysis, the enzyme encoded by CsSesquiTPS6a produces exclusively beta-caryophyllene, while the enzyme encoded by CsSesquiTPS6b and CsSesquiTPS7 render higher amounts of beta-caryophyllene (about 73% or 75% of total, respectively) together with alpha-humulene (about 23% or 25% of total, respectively) | Citrus sinensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A256E | site-directed mutagenesis of an alpha-helix residue disturbs the facing of the N-terminal loop | Citrus sinensis |
| D319G | site-directed mutagenesis, the mutation prevents the interaction of this residue with one of the Mg2+ atoms | Citrus sinensis |
| E41D | site-directed mutagenesis of an alpha-helix residue facing the N-terminal loop, the mutation can compensate the structural difference between enzymes CsSesquiTPS7 and CsSesquiTPS6b, both producing beta-caryophyllene and alpha-humulene | Citrus sinensis |
| I260R | site-directed mutagenesis of an alpha-helix residue disturbs the facing of the N-terminal loop | Citrus sinensis |
| T44P | site-directed mutagenesis of an alpha-helix residue facing the N-terminal loop, the mutation can compensate the structural difference between enzymes CsSesquiTPS7 and CsSesquiTPS6b, both producing beta-caryophyllene and alpha-humulene | Citrus sinensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, interaction between Mg2+ atoms and binding residues, structure overview | Citrus sinensis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | Citrus sinensis | - |
(+)-beta-caryophyllene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrus sinensis | A0A067G476 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
| Citrus sinensis | A0A290U6M8 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
| Citrus sinensis | A0A290U6M9 | i.e. Citrus aurantium var. sinensis, pineapple sweet orange | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Citrus sinensis | - |
| leaf | - |
Citrus sinensis | - |
| additional information | in orange tissues, the sesquiterpene profile is qualitatively rich. Overall, up to 25 sesquiterpenes are identified in total, overview. Small amounts of beta-caryophyllene are detected in all samples, while alpha-copaene, beta-elemene, alpha-humulene, and alpha-selinene are emitted by all tissues except young leaves. Beside this, each tissue shows a characteristic sesquiterpene emission profile. Major sesquiterpenes emitted by adult leaves are beta-elemene and beta-caryophyllene, while flowers emit nerolidol, absent from most tissues analyzed. In fruit tissues, valencene emission contributes greatly to total emitted volatiles, in much more extent in peel than in pulp | Citrus sinensis | - |
| additional information | in orange tissues, the sesquiterpene profile is qualitatively rich. Overall, up to 25 sesquiterpenes are identified in total, overview. Small amounts of beta-caryophyllene are detected in all samples, while alpha-copaene, beta-elemene, alpha-humulene, and alpha-selinene are emitted by all tissues except young leaves. Beside this, each tissue shows a characteristic sesquiterpene emission profile. Major sesquiterpenes emitted by adult leaves are beta-elemene and beta-caryophyllene, while flowers emitt nerolidol, absent from most tissues analyzed. In fruit tissues, valencene emission contributes greatly to total emitted volatiles, in much more extent in peel than in pulp | Citrus sinensis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (2E,6E)-farnesyl diphosphate | - |
Citrus sinensis | (+)-beta-caryophyllene + diphosphate | - |
? | |
| additional information | GC-MS analysis is used for compound identification | Citrus sinensis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-caryophyllene synthase | - |
Citrus sinensis |
| CsSesquiTPS6a | - |
Citrus sinensis |
| CsSesquiTPS6b | - |
Citrus sinensis |
| CsSesquiTPS6c | - |
Citrus sinensis |
| SQS6a | - |
Citrus sinensis |
| SQS6b | - |
Citrus sinensis |
| SQS7 | - |
Citrus sinensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the terpene synthase (TPS) family | Citrus sinensis |
| additional information | three-dimensional structure homology modelling using the crystal structure of the delta-cadinene synthase from Gossypium. The functional C-terminal domain consists of 14 alpha-helices forming a central cavity and the amino terminal domain formed by two 10 antiparallel alpha-helices. The aspartate-rich motifs DDXXD (located on helix-alpha-13) and NSE/DTE motif (located helix alpha-20) are both involved in binding a trio of divalent magnesium ions, co-orientated to the catalytic pocket | Citrus sinensis |
| additional information | three-dmensional structure homology modelling using the crystal structure of the delta-cadinene synthase from Gossypium. The functional C-terminal domain consists of 14 alpha-helices forming a central cavity and the amino terminal domain formed by two 10 antiparallel alpha-helices. The aspartate-rich motifs DDXXD (located on helix-alpha-13) and NSE/DTE motif (located helix alpha-20) are both involved in binding a trio of divalent magnesium ions, co-orientated to the catalytic pocket | Citrus sinensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
