Literature summary for 4.2.3.87 extracted from

Deguerry, F.; Pastore, L.; Wu, S.; Clark, A.; Chappell, J.; Schalk, M.
The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated with a limited number of sesquiterpene synthases (2006), Arch. Biochem. Biophys., 454, 123-136.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Pogostemon cablin

Inhibitors

Inhibitors	Comment	Organism	Structure
Mn2+	concentrations higher than 0.1 mM are inhibitory	Pogostemon cablin

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00445	-	(2E,6E)-farnesyl diphosphate	pH 7.0, 30°C	Pogostemon cablin

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Mg2+ is slightly preferred to Mn2+ with maximum activity observed at 1 mM Mg2+ and dropping to less than 10% at 0.1 mM Mg2+	Pogostemon cablin
Mn2+	Mn2+ can substitute for Mg2+ at a concentration of 0.1 mM	Pogostemon cablin

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
55000	-	x * 55000, SDS-PAGE	Pogostemon cablin
64000	-	x * 64000, calculated from amino acid sequence	Pogostemon cablin

Organism

Organism	UniProt	Comment	Textmining
Pogostemon cablin	Q49SP3	patchouli	-

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ affinity column chromatography	Pogostemon cablin

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Pogostemon cablin	alpha-guaiene + diphosphate	13% yield with recombinant enzyme	?

Subunits

Subunits	Comment	Organism
?	x * 55000, SDS-PAGE	Pogostemon cablin
?	x * 64000, calculated from amino acid sequence	Pogostemon cablin

Synonyms

Synonyms	Comment	Organism
Pat177	recombinant enzyme	Pogostemon cablin
patchoulol synthase	-	Pogostemon cablin

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Pogostemon cablin

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	40	at 30°C, the enzymatic activity is less than 60% of the activity measured at 40°C and at 20°C this value is 30%	Pogostemon cablin

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.00043	-	(2E,6E)-farnesyl diphosphate	pH 7.0, 30°C	Pogostemon cablin

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	7.5	-	Pogostemon cablin

pH Range

pH Minimum	pH Maximum	Comment	Organism
7	9	75% of the maximum activity is recorded at pH 8.0-9.0. Enzyme activity is dramatically reduced below pH 7.0, but is rather stable above this pH value	Pogostemon cablin

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Release 2023.1 (January 2023)