Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solidago canadensis |
Protein Variants | Comment | Organism |
---|---|---|
N303D | mimicking of corresponding amino acid in (-)-germacrene D synthase Sc19. Kinetic data similar to wild-type | Solidago canadensis |
N303E | less than 0.25% of wild-type activity | Solidago canadensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
(2E,6E)-farnesyl diphosphate | 25°C, pH not specified in the publication | Solidago canadensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required. Bi-dentate Mg2+-binding by residue 303 is not obligatory | Solidago canadensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64802 | - |
x * 64802, calculated | Solidago canadensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solidago canadensis | Q70EZ7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Solidago canadensis | (+)-germacrene D + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 64802, calculated | Solidago canadensis |
Synonyms | Comment | Organism |
---|---|---|
Sc11 | - |
Solidago canadensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
(2E,6E)-farnesyl diphosphate | 25°C, pH not specified in the publication | Solidago canadensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.7 | - |
(2E,6E)-farnesyl diphosphate | 25°C, pH not specified in the publication | Solidago canadensis |