Literature summary for 4.2.3.77 extracted from

Prosser, I.; Altug, I.G.; Phillips, A.L.; Koenig, W.A.; Bouwmeester, H.J.; Beale, M.H.
Enantiospecific (+)- and (-)-germacrene D synthases, cloned from goldenrod, reveal a functionally active variant of the universal isoprenoid-biosynthesis aspartate-rich motif (2004), Arch. Biochem. Biophys., 432, 136-144.

Search for more literature for 4.2.3.77

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Solidago canadensis

Protein Variants

Protein Variants	Comment	Organism
N303D	mimicking of corresponding amino acid in (-)-germacrene D synthase Sc19. Kinetic data similar to wild-type	Solidago canadensis
N303E	less than 0.25% of wild-type activity	Solidago canadensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.003	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required. Bi-dentate Mg2+-binding by residue 303 is not obligatory	Solidago canadensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
64802	-	x * 64802, calculated	Solidago canadensis

Organism

Organism	UniProt	Comment	Textmining
Solidago canadensis	Q70EZ7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Solidago canadensis	(+)-germacrene D + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 64802, calculated	Solidago canadensis

Synonyms

Synonyms	Comment	Organism
Sc11	-	Solidago canadensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.02	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.7	-	(2E,6E)-farnesyl diphosphate	25°C, pH not specified in the publication	Solidago canadensis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)