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Literature summary for 4.2.3.70 extracted from

  • Albertsen, L.; Chen, Y.; Bach, L.S.; Rattleff, S.; Maury, J.; Brix, S.; Nielsen, J.; Mortensen, U.H.
    Diversion of flux toward sesquiterpene production in Saccharomyces cerevisiae by fusion of host and heterologous enzymes (2011), Appl. Environ. Microbiol., 77, 1033-1040.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.70

Application

Application Comment Organism
synthesis construction of several fusion protein variants in which farnesyl diphosphate synthase of yeast has been coupled to patchoulol synthase of plant origin. Expression of the fusion proteins in Saccharomyces cerevisiae increases the production of patchoulol up to 2-fold. The fusion strategy can be used in combination with traditional metabolic engineering to further increase the production of patchoulol Pogostemon cablin
synthesis use of a bifunctional enzymatic chimera composed as a fusion between farnesyl diphosphate synthase from yeast and patchoulol synthase from patchouli improves patchoulol production compared to that with the use of free enzymes up to 2fold. The effect is additive to improvements obtained by traditional metabolic engineering Pogostemon cablin

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Pogostemon cablin

Protein Variants

Protein Variants Comment Organism
additional information construction of several fusion protein variants in which farnesyl diphosphate synthase of yeast has been coupled to patchoulol synthase of plant origin. patchoulol synthase can be extended N- and C-terminally with farnesyl diphosphate synthase and retain activity in vivo. Expression of the fusion proteins in Saccharomyces cerevisiae increases the production of patchoulol up to 2-fold Pogostemon cablin
additional information use of a bifunctional enzymatic chimera composed as a fusion between farnesyl diphosphate synthase from yeast and patchoulol synthase from patchouli improves patchoulol production compared to that with the use of free enzymes up to 2fold. The effect is additive to improvements obtained by traditional metabolic engineering Pogostemon cablin

Organism

Organism UniProt Comment Textmining
Pogostemon cablin
-
-
-
Pogostemon cablin Q49SP3
-
-

Synonyms

Synonyms Comment Organism
PTS
-
 Pogostemon cablin
TPS
-
 Pogostemon cablin