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Literature summary for 4.2.3.69 extracted from

  • Wu, S.; Schoenbeck, M.A.; Greenhagen, B.T.; Takahashi, S.; Lee, S.; Coates, R.M.; Chappell, J.
    Surrogate splicing for functional analysis of sesquiterpene synthase genes (2005), Plant Physiol., 138, 1322-1333.
    View publication on PubMedView publication on EuropePMC
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Application

Application Comment Organism
analysis method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host provides for direct functional testing of the encoded gene product Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Petunia hybrida infiltrated with Agrobacterium tumefaciens harboring the enzyme gene Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate Arabidopsis thaliana
-
 alpha-barbatene + diphosphate main products, 27.3% alpha-barbatene, 17.8% thujopsene, and 9.9% b-chamigrene, plus 13 minor products ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q4KSH9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate
-
 Arabidopsis thaliana alpha-barbatene + diphosphate main products, 27.3% alpha-barbatene, 17.8% thujopsene, and 9.9% b-chamigrene, plus 13 minor products ?
additional information no substrate: geranyl diphosphate, geranylgeranyl diphosphate Arabidopsis thaliana ?
-
 ?