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Literature summary for 4.2.3.66 extracted from

  • Belingheri, L.; Cartayrade, A.; Pauly, G.; Gleizes, M.
    Partial purification and properties of the sesquiterpene beta-selinene cyclase from Citrofortunella mitis fruits (1992), Plant Sci., 84, 129-136.
No PubMed abstract available
Search for more literature for 4.2.3.66

Inhibitors

Inhibitors Comment Organism Structure
Mn2+ inhibitory above 0.2 mM, stimulation below Citrus x microcarpa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 (2E,6E)-farnesyl diphosphate pH 7.6, 28°C Citrus x microcarpa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximum activity at 10 mM Citrus x microcarpa
Mn2+ about 60% of the activity with Mg2+, inhibitory above 0.2 mM Citrus x microcarpa
additional information presence of divalent metal ion required, Mg2+ is preferred Citrus x microcarpa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
 gel filtration Citrus x microcarpa

Organism

Organism UniProt Comment Textmining
Citrus x microcarpa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Citrus x microcarpa

Source Tissue

Source Tissue Comment Organism Textmining
flavedo
-
 Citrus x microcarpa
-
fruit flavedo of immature fruit Citrus x microcarpa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5180
-
 pH 7.6, 28°C Citrus x microcarpa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Citrus x microcarpa beta-selinene + diphosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Citrus x microcarpa

pI Value

Organism Comment pI Value Maximum pI Value
Citrus x microcarpa isoelectric focusing
-
 6