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Literature summary for 4.2.3.61 extracted from

  • Rising, K.; Starks, C.; Noel, J.; Chappell, J.
    Demonstration of germacrene A as an intermediate in 5-epi-aristolochene synthase catalysis (2000), J. Am. Chem. Soc., 122, 1861-1866.
No PubMed abstract available
Search for more literature for 4.2.3.61

Protein Variants

Protein Variants Comment Organism
Y520F 3% of wild-type catalytic efficiency, reaction prooduct is germacrene A Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 30°C Nicotiana tabacum
0.0081
-
 (2E,6E)-farnesyl diphosphate mutant Y520F, pH 7.5, 30°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q40577
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate reaction proceeds via geracrene A as an intermediate. Proton donation by residue Y520 is responsible for the activation of germacrene A to a eudesmane cation Nicotiana tabacum (+)-5-epiaristolochene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
EAS3
-
 Nicotiana tabacum
EAS4
-
 Nicotiana tabacum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 (2E,6E)-farnesyl diphosphate mutant Y520F, pH 7.5, 30°C Nicotiana tabacum
0.048
-
 (2E,6E)-farnesyl diphosphate wild-type, pH 7.5, 30°C Nicotiana tabacum