Literature summary for 4.2.3.61 extracted from

Wu, S.; Schoenbeck, M.A.; Greenhagen, B.T.; Takahashi, S.; Lee, S.; Coates, R.M.; Chappell, J.
Surrogate splicing for functional analysis of sesquiterpene synthase genes (2005), Plant Physiol., 138, 1322-1333.

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Application

Application	Comment	Organism
analysis	method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host provides for direct functional testing of the encoded gene product	Nicotiana tabacum

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Petunia hybrida infiltrated with Agrobacterium tumefaciens harboring the enzyme gene	Nicotiana tabacum

Organism

Organism	UniProt	Comment	Textmining
Nicotiana tabacum	Q7X9A3	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Nicotiana tabacum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Nicotiana tabacum	5-epi-aristolochene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
g110	-	Nicotiana tabacum

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Release 2023.1 (January 2023)