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Literature summary for 4.2.3.6 extracted from

  • Cane, D.E.; Chiu, H.T.; Liang, P.H.; Anderson, K.S.
    Pre-steady-state kinetic analysis of the trichodiene synthase reaction pathway (1997), Biochemistry, 36, 8332-8339.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D101E Km-value for farneyl diphosphate: 124 nM Fusarium sporotrichioides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000078
-
farnesyl diphosphate 15°C, pH 7.8 Fusarium sporotrichioides
0.00009
-
farnesyl diphosphate 30°C, pH 7.8 Fusarium sporotrichioides
0.000124
-
farnesyl diphosphate 30°C, pH 7.8, mutant D101E Fusarium sporotrichioides

Organism

Organism UniProt Comment Textmining
Fusarium sporotrichioides
-
-
-

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate mechanism, kinetics, isomerization of farnesyl diphosphate to nerolidyl diphosphate is the rate limiting step Fusarium sporotrichioides