Application | Comment | Organism |
---|---|---|
biofuel production | acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce b-caryophyllene from reproducible sources. Contribution by recombinant production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain | Arabidopsis thaliana |
biofuel production | acute demand for high-density fuels has provided the impetus to pursue biosynthetic methods to produce b-caryophyllene from reproducible sources. Contribution by recombinant production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain | Artemisia annua |
Cloned (Comment) | Organism |
---|---|
codon-optimized QHS1 gene, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH | Artemisia annua |
expressed in Escherichia coli strain YJM59 | Arabidopsis thaliana |
expressed in Escherichia coli strain YJM59 | Artemisia annua |
expressed in Escherichia coli strain YJM59 | Zea perennis |
gene TPS21, recombinant overexpression in Escherichia coli strain YJM59, coexpression with geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene) from plasmid pACY-mvaE-mvaS-QHS1-GPPS2-G6PDH | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1-deoxy-D-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways are employed. Geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene), and beta-caryophyllene synthase genes are co-overexpressed in the engineered strain. The final genetically modified strain, YJM59, produces 220 mg/l of beta-caryophyllene in flask culture. Evaluation of fed-batch fermentation for the production of beta-caryophyllene. After induction for 60 h, the YJM59 strain produces beta-caryophyllene at a concentration of 1520 mg/l. The volumetric production fermented in the aerobic fed-batch is 0.34 mg/(l/h/OD600) and the conversion efficiency of glucose to beta-caryophyllene (gram to gram) is 1.69%. Method evaluation with beta-caryophyllene synthases from different origins, QHS1 from Artemisia annua is the most effective of the three enzymes, compared to TPS21 from Arabidopsis thaliana and TPS23 from Zea perennis. Substrate channeling | Arabidopsis thaliana |
additional information | production of beta-caryophyllene by assembling a biosynthetic pathway in an engineered Escherichia coli strain of which phosphoglucose isomerase gene has been deleted. The 1-deoxy-D-xylulose 5-phosphate (DXP) or heterologous mevalonate (MVA) pathways are employed. Geranyl diphosphate synthase (GPPS2 gene from Abies grandis), glucose-6-phosphate dehydrogenase (G6PDH gene), and beta-caryophyllene synthase genes are co-overexpressed in the engineered strain. The final genetically modified strain, YJM59, produces 220 mg/l of beta-caryophyllene in flask culture. Evaluation of fed-batch fermentation for the production of beta-caryophyllene. After induction for 60 h, the YJM59 strain produces beta-caryophyllene at a concentration of 1520 mg/l. The volumetric production fermented in the aerobic fed-batch is 0.34 mg/(l/h/OD600) and the conversion efficiency of glucose to beta-caryophyllene (gram to gram) is 1.69%. Method evaluation with beta-caryophyllene synthases from different origins, QHS1 from Artemisia annua is the most effective of the three enzymes, compared to TPS21 from Arabidopsis thaliana and TPS23 from Zea perennis. Substrate channeling | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Arabidopsis thaliana | - |
(-)-beta-caryophyllene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Artemisia annua | - |
(-)-beta-caryophyllene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Zea perennis | - |
(-)-beta-caryophyllene + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q84UU4 | - |
- |
Artemisia annua | Q8SA63 | - |
- |
Zea perennis | B2C4D8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Arabidopsis thaliana | (-)-beta-caryophyllene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Artemisia annua | (-)-beta-caryophyllene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | - |
Zea perennis | (-)-beta-caryophyllene + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-humulene/(-)-(E)-beta-caryophyllene synthase | UniProt | Arabidopsis thaliana |
QHS1 | - |
Artemisia annua |
TPS21 | - |
Arabidopsis thaliana |
tps23 | - |
Zea perennis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Arabidopsis thaliana |
30 | - |
in vivo assay at | Artemisia annua |
General Information | Comment | Organism |
---|---|---|
metabolism | beta-caryophyllene is produced via the mevalonate (MVA)-mediated pathway, overview | Arabidopsis thaliana |
metabolism | beta-caryophyllene is produced via the mevalonate (MVA)-mediated pathway, overview | Artemisia annua |