Literature summary for 4.2.3.57 extracted from

Salvagnin, U.; Carlin, S.; Angeli, S.; Vrhovsek, U.; Anfora, G.; Malnoy, M.; Martens, S.
Homologous and heterologous expression of grapevine E-(beta)-caryophyllene synthase (VvGwECar2) (2016), Phytochemistry, 131, 76-83 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21, Rosetta and RIPL strains and in Arabidopsis thaliana	Vitis vinifera

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0314	-	(2E,6E)-farnesyl diphosphate	at pH 7.8 and 30°C	Vitis vinifera

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Vitis vinifera	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	Vitis vinifera	-	(E)-beta-caryophyllene + alpha-humulene + germacrene + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	E5GAF4	-	-

Purification (Commentary)

Purification (Comment)	Organism
Profinity IMAC resin column chromatography	Vitis vinifera

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(2E,6E)-farnesyl diphosphate	-	Vitis vinifera	(E)-beta-caryophyllene + alpha-humulene + germacrene + diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
E-(beta)-caryophyllene synthase	-	Vitis vinifera
GwECar2	-	Vitis vinifera

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.19	-	(2E,6E)-farnesyl diphosphate	at pH 7.8 and 30°C	Vitis vinifera

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	-	Vitis vinifera

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
6.1	-	(2E,6E)-farnesyl diphosphate	at pH 7.8 and 30°C	Vitis vinifera

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Release 2023.1 (January 2023)