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Literature summary for 4.2.3.57 extracted from

  • Yang, J.; Nie, Q.
    Engineering Escherichia coli to convert acetic acid to beta-caryophyllene (2016), Microb. Cell Fact., 15, 74 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene QHS1, the optimized QHS1 gene is expressed in Escherichia coli strain YJM63 and BL21(DE3), coexpression with genes ACS, GPPS2, and AACS to construct a beta-caryophyllene overroduction strain Artemisia annua

Protein Variants

Protein Variants Comment Organism
additional information in a robustly acetate-utilizing Escherichia coli strain, acetyl-CoA synthase (ACS), Artemisia annua beta-caryophyllene synthase (QHS1) and geranyl diphosphate synthase (GPPS2) are co-expressed to establish the engineered strain converting acetic acid to beta-caryophyllene. To further enhance beta-caryophyllene production from acetic acid, the heterologous mevalonate pathway is introduced into the cells. Acetoacetyl-CoA synthase (AACS) is also expressed in the cells to increase the precursor acetoacetyl-CoA and accordingly resulted in the increase of beta-caryophyllene. The final genetically modified strain, YJM67, accumulates the production of biomass and beta-caryophyllene up to 12.6 and 1.05 g/l during 72 h, respectively, with a specific productivity of 1.15 mg/h/g dry cells, and the conversion efficiency of acetic acid to beta-caryophyllene (gram to gram) reaching 2.1%. The yield of beta-caryophyllene on acetic acid of this strain also reaches about 5.6 % of the theoretical yield. A pH-coupled HAc fed-batch fermentation in large scale production of beta-caryophyllene Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Artemisia annua stereochemistry not specified in the publication beta-caryophyllene + diphosphate
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 ?

Organism

Organism UniProt Comment Textmining
Artemisia annua Q8SA63
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate stereochemistry not specified in the publication Artemisia annua beta-caryophyllene + diphosphate
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 ?

Synonyms

Synonyms Comment Organism
beta-caryophyllene synthase
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 Artemisia annua
QHS1
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 Artemisia annua

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Artemisia annua