Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.57 extracted from

  • Jin, Z.; Kwon, M.; Lee, A.R.; Ro, D.K.; Wungsintaweekul, J.; Kim, S.U.
    Molecular cloning and functional characterization of three terpene synthases from unripe fruit of black pepper (Piper nigrum) (2018), Arch. Biochem. Biophys., 638, 35-40 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene TPS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS), illumina transcriptome sequencing of unripe peppercorn identifiying sesquiterpene synthases (sesqui-TPSs), recombinant expression of His-tagged enzyme TPS1 in Escherichia coli strain Rosetta 2(DE3) Piper nigrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten steady-state kinetics Piper nigrum
0.0211
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, 30°C, recombinant His-tagged enzyme Piper nigrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(2E,6E)-farnesyl diphosphate Piper nigrum the enzyme stereochemistry is not determined in the publication beta-caryophyllene + diphosphate
-
?
additional information Piper nigrum the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS) ?
-
?

Organism

Organism UniProt Comment Textmining
Piper nigrum A0A1V0E492 from garden of Prince of Songkla University, Thailand
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme TPS1 from Escherichia coli strain Rosetta 2(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration Piper nigrum

Reaction

Reaction Comment Organism Reaction ID
(2E,6E)-farnesyl diphosphate = (-)-beta-caryophyllene + diphosphate possible mechanism of sesquiterpene synthases from Piper nigrum, the reaction mechanism includes a 1,10-ring closure, overview Piper nigrum

Source Tissue

Source Tissue Comment Organism Textmining
fruit unripe peppercorn is subjected to the Illumina transcriptome sequencing, young, unripe, and ripe fruits. GC/MS profiling of terpenes from immature black pepper fruit, accumulation of beta-caryophyllene, the major sesquiterpene in peppercorn, changes from 46% to 70% as fruits ripen Piper nigrum
-
leaf low level Piper nigrum
-
stem high level Piper nigrum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate the enzyme stereochemistry is not determined in the publication Piper nigrum beta-caryophyllene + diphosphate
-
?
(2E,6E)-farnesyl diphosphate the enzyme stereochemistry is not determined in the publication, the reaction mechanism includes a 1,10-ring closure Piper nigrum beta-caryophyllene + diphosphate
-
?
additional information the enzyme encoded by gene TPS1 produces beta-caryophyllene as a main product and humulene as a minor compound, and thus is named caryophyllene synthase (PnCPS) Piper nigrum ?
-
?
additional information steady-state kinetic parameters are determined using malachite green phosphate assay Piper nigrum ?
-
?

Subunits

Subunits Comment Organism
? x * 64900, about, sequence calculation Piper nigrum

Synonyms

Synonyms Comment Organism
More cf. EC 4.2.3.89 Piper nigrum
PnCPS
-
Piper nigrum
PnTPS1
-
Piper nigrum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Piper nigrum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.455
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, 30°C, recombinant His-tagged enzyme Piper nigrum

General Information

General Information Comment Organism
evolution the cyclization process catalyzed by PnTPSs follows a 1,10-closure mechanism assuming that the present PnTPSs have evolved from a common ancestor Piper nigrum
metabolism GC/MS profiling of terpenes from immature black pepper fruit, accumulation of beta-caryophyllene, the major sesquiterpene in peppercorn, changes from 46% to 70% as fruits ripen Piper nigrum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
21.6
-
(2E,6E)-farnesyl diphosphate pH not specified in the publication, 30°C, recombinant His-tagged enzyme Piper nigrum