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Literature summary for 4.2.3.56 extracted from

  • Little, D.B.; Croteau, R.B.
    Alteration of product formation by directed mutagenesis and truncation of the multiple-product sesquiterpene synthases delta-selinene synthase and gamma-humulene synthase (2002), Arch. Biochem. Biophys., 402, 120-135.
    View publication on PubMed
Search for more literature for 4.2.3.56

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Abies grandis

Protein Variants

Protein Variants Comment Organism
D343A the mutation results in severely impaired activity for olefin production (estimated kcat reduction by more than 3000fold), the mutant produces 27.9% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 12.9% gamma-humulene (the main product of the wild type enzyme) Abies grandis
D343E the mutant produces 34.4% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme), the mutant is capable of producing sesquiterpenols (61% nerolidol and 14% bisabolol, very similar to wild type distribution) at a rate comparable to that of olefin formation by the wild type enzyme Abies grandis
D488A the mutant produces 40% sativene from (2E,6E)-farnesyl diphosphate as the main product and no gamma-humulene (the main product of the wild type enzyme) Abies grandis
D488E the mutant produces 19.6% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 6.0% gamma-humulene (the main product of the wild type enzyme), olefin synthase activity is reduced to 3% of wild type enzyme Abies grandis
D488N the mutant produces 21.7% sativene from (2E,6E)-farnesyl diphosphate as the main product and only 18.7% gamma-humulene (the main product of the wild type enzyme) Abies grandis
D570N the mutant produces 13.1% sativene from (2E,6E)-farnesyl diphosphate as the main product and 29.3% gamma-humulene (the main product of the wild type enzyme) Abies grandis
Y566F the mutant produces 42.6% gamma-humulene (the main product of the wild type enzyme) Abies grandis

Organism

Organism UniProt Comment Textmining
Abies grandis O64405 grand fir
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Purification (Commentary)

Purification (Comment) Organism
hydroxyapatite column chromatography followed by gradient elution from an anion-exchange matrix Abies grandis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
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 Abies grandis gamma-humulene + diphosphate gamma-humulene is the main product (30.9% yield) of the wild type enzyme ?
additional information besides gamma-humulene (30.9% yield), gamma-humulene synthase produces 18.9% siberene, 11.4% longifolene, 7.4% beta-bisabolene, 4.3% sativene, 4.1% alpha-longipinene, 3.0% beta-farnesene, 2.6% alpha-ylangene, 2.1% beta-gurjunene, 2.0% gamma-bisabolene, 1.7% beta-longipinene, 1.3% alpha-bisaolene, 1.3% alpha-himachalene, 1.1% gamma-himachalene, 1.0% cyclosativene, 0.6% beta-ylangene, and 0.2% beta-cubebene Abies grandis ?
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 ?

Synonyms

Synonyms Comment Organism
gamma-humulene synthase
-
 Abies grandis
gammaHS
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 Abies grandis