Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
D526N | mutation inactivates the enzyme completely | Zea mays |
Y522F | mutation reduces the production of (S)-beta-macrocarpene to trace amounts, the enzyme forms (S)-beta-bisabolene almost exclusively, the overall activity of the mutated enzyme is dramatically reduced | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
(2E,6E)-farnesyl diphosphate | 30°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Zea mays | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mg2+ measured with 0.01 mM farnesyl diphosphate: 0.131 mM | Zea mays | |
Mn2+ | TPS6 requires a divalent metal ion, with Mg2+ and Mn2+ being effective, optimum activity with 5 mM Mg2+ and 5 mM Mn2+. Although the Km for Mn2+ is significantly lower than for Mg2+, the enzyme is more likely to operate with a Mg2+ cofactor in planta, because the concentration of Mg2+ in plant cells is about 2 orders of magnitudes higher than Mn2+. In the presence of Mn2+, the product spectrum of TPS6 is shifted toward an increased production of (S)-beta bisabolene and a decreased production of (S)-beta-macrocarpene. KM-value for Mn2+ measured with 0.01 mM farnesyl diphosphate: 0.0234 mM | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | Zea mays | the enzyme may be involved in plant defense | (S)-beta-bisabolene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | Zea mays | the enzyme may be involved in plant defense | (S)-beta-bisabolene + diphosphate | i.e. ((4S)-1-methyl-4-(5-methyl-1-methylidenehex-4-en-1-yl)cyclohexene | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | Q1EG72 | L. variety B73 | - |
Zea mays | Q5GJ60 | L. variety B73 | - |
Purification (Comment) | Organism |
---|---|
partial | Zea mays |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate = (S)-beta-bisabolene + diphosphate | (S)-beta-bisabolene is a stable intermediate. (S)-beta-Macrocarpene formation requires a protonation of the intermediate | Zea mays | |
(2E,6E)-farnesyl diphosphate = (S)-beta-bisabolene + diphosphate | The synthesis of (S)-beta-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-beta-bisabolene. The second steps is the isomerization to (S)-beta-macrocarpene (c.f. EC 5.5.1.a, (S)-beta-macrocarpene synthase). The enzyme requires Mg2+ or Mn2+ for activity. | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | the enzyme is predominantly active in the roots | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate | - |
Zea mays | (S)-beta-bisabolene + diphosphate | The synthesis of (S)-beta-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-beta-bisabolene. The second step is the isomerization to (S)-beta-macrocarpene ((S)-beta-macrocarpene synthase). Product is identified by GC-MS | ? | |
(2E,6E)-farnesyl diphosphate | the enzyme may be involved in plant defense | Zea mays | (S)-beta-bisabolene + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate | the enzyme may be involved in plant defense | Zea mays | (S)-beta-bisabolene + diphosphate | i.e. ((4S)-1-methyl-4-(5-methyl-1-methylidenehex-4-en-1-yl)cyclohexene | ? | |
(2Z,6E)-farnesyl diphosphate | - |
Zea mays | (S)-beta-bisabolene + diphosphate | The synthesis of (S)-beta-macrocarpene from (2E,6E)-farnesyl diphosphate proceeds in two steps. The first step is the cyclization to (S)-beta-bisabolene. The second step is the isomerization to (S)-beta-macrocarpene ((S)-beta-macrocarpene synthase). Product is identified by GC-MS | ? | |
additional information | no activity with geranylgeranyl diphosphate. TPS6 accepts the monoterpene precursor geranyl diphosphate but the monoterpene products are produced at a lower velocity | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TPS11 | - |
Zea mays |
TPS6 | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
in presence of 5 mM Mg2+ | Zea mays |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.6 | half-maximal activity at pH 6.2 and at pH 8.6, in presence of 5 mM Mg2+. Within a pH range from 5.0 to 8.0, the major product is (S)-beta-macrocarpene, but higher pH values favor the formation of (S)-beta-bisabolene | Zea mays |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme may be involved in plant defense | Zea mays |