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Literature summary for 4.2.3.5 extracted from
- Purification and properties of chorismate synthase from Bacillus subtilis (1978), J. Biol. Chem., 253, 4993-4998.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus subtilis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| together with NADPH dependent flavin reductase | Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate | combination with flavin reductase activity | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.92 | - |
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.05 mM Tris-HCl, pH 7.5, 0.01 mM FMN, 10% glycerol, 3 mM mercaptoethanol, 4 months | Bacillus subtilis |
| 4°C, 2 weeks | Bacillus subtilis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | x * 24000, SDS-PAGE | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| flavins | required, reduced forms e.g. FMN, FAD | Bacillus subtilis | |
| NADPH | required | Bacillus subtilis | |
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