Literature summary for 4.2.3.5 extracted from

White, P.J.; Millar, G.; Coggins, J.R.
The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa (1988), Biochem. J., 251, 313-322.

Search for more literature for 4.2.3.5

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	4 * 38000, SDS-PAGE	Escherichia coli
50000	-	4 * 50000, SDS-PAGE	Neurospora crassa
144000	-	gel filtration after cross-linkage with dimethyl suberimidate	Escherichia coli
198000	-	gel filtration after cross-linkage with dimethyl suberimidate	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Neurospora crassa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli
-	Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
14.8	-	-	Escherichia coli
32.1	-	-	Neurospora crassa

Storage Stability

Storage Stability	Organism
-20°C, 50 mM Tris-HCl buffer, pH 7.5, 0.4 mM dithiothreitol, 50% glycerol	Escherichia coli
-20°C, 50 mM Tris-HCl buffer, pH 7.5, 0.4 mM dithiothreitol, 50% glycerol	Neurospora crassa

Subunits

Subunits	Comment	Organism
tetramer	4 * 50000, SDS-PAGE	Neurospora crassa
tetramer	4 * 38000, SDS-PAGE	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)