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Literature summary for 4.2.3.48 extracted from

  • Arimura, G.; Garms, S.; Maffei, M.; Bossi, S.; Schulze, B.; Leitner, M.; Mithöfer, A.; Boland, W.
    Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling (2008), Planta, 227, 453-464.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
subcloned into the pHis8–3 expression vector and transformed into Escherichia coli BL21-CodonPlus(DE3) Medicago truncatula

Organism

Organism UniProt Comment Textmining
Medicago truncatula Q5UB06 wild-type cv. Jemalong
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate + H2O MtTPS3 produces linalool at 5% of the rate of (E)-nerolidol. Using geranylgeranyl diphosphate the enzyme fails to produce diterpenoids. The recombinant MtTPS3 generates the diterpene geranyllinalool when supplied with geranylgeranyl diphosphate (65% of the rate of (E)-nerolidol) Medicago truncatula (3S,6E)-nerolidol + diphosphate
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Synonyms

Synonyms Comment Organism
MtTPS3
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 Medicago truncatula
nerolidol/geranyl linalool synthase
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 Medicago truncatula

Expression

Organism Comment Expression
Medicago truncatula MtTPS3 activity is induced by jasmonic acid ((E)-4,8-dimethyl-1,3,7-nonatriene) and by jasmonic acid + (E)-nerolidol. Feeding Beet armyworm raises the expression level of MtTPS3 in wild-type and skl plants in the same way, but levels of (E)-nerolidol and (E)-4,8-dimethyl-1,3,7-nonatriene formation are higher in wild-type plants, suggesting that ethylene might have a post-transcriptional impact on the MtTPS3 protein up