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Literature summary for 4.2.3.47 extracted from

  • Faraldos, J.A.; Gonzalez, V.; Li, A.; Yu, F.; Koeksal, M.; Christianson, D.W.; Allemann, R.K.
    Probing the mechanism of 1,4-conjugate elimination reactions catalyzed by terpene synthases (2012), J. Am. Chem. Soc., 134, 20844-20848.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.47

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mentha x piperita

Inhibitors

Inhibitors Comment Organism Structure
(2E,6E)-2-fluorofarnesyl diphosphate competitive Mentha x piperita
15-fluorofarnesyl diphosphate
-
 Mentha x piperita
15-trifluorofarnesyl diphosphate
-
 Mentha x piperita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 (2Z,6E)-2-fluorofarnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.018
-
 (2E,6E)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.025
-
 (3RS)-trans-nerolidyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita

Organism

Organism UniProt Comment Textmining
Mentha x piperita
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesyl diphosphate
-
 Mentha x piperita (E)-beta-farnesene + diphosphate
-
 ?
(2Z,6E)-2-fluorofarnesyl diphosphate
-
 Mentha x piperita (E)-beta-2-fluorofarnesene + diphosphate
-
 ?
(3RS)-trans-nerolidyl diphosphate
-
 Mentha x piperita (E)-beta-farnesene + diphosphate
-
 ?
additional information stepwise reaction mechanism through enzyme-bound allylic cationic intermediates. The elimination reaction can proceed via the enzyme-bound intermediate trans-nerolidyl diphosphate Mentha x piperita ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
 (2Z,6E)-2-fluorofarnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.023
-
 (3RS)-trans-nerolidyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.028
-
 (2E,6E)-farnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
 (2E,6E)-2-fluorofarnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.0016
-
 15-trifluorofarnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita
0.0023
-
 15-fluorofarnesyl diphosphate pH not specified in the publication, temperature not specified in the publication Mentha x piperita