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Literature summary for 4.2.3.44 extracted from

  • Chen, R.; Bu, Y.; Ren, J.; Pelot, K.; Hu, X.; Diao, Y.; Chen, W.; Zerbe, P.; Zhang, L.
    Discovery and modulation of diterpenoid metabolism improves glandular trichome formation, artemisinin production and stress resilience in Artemisia annua (2021), New Phytol., 230, 2387-2403.
    View publication on PubMed
Search for more literature for 4.2.3.44

Cloned(Commentary)

Cloned (Comment) Organism
transient heterologous expression of the enzyme in Nicotiana benthaminana via transfection with Agrobacterium strain GV3101 quantitative real-time PCR enzyme expression analysis Artemisia annua

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Artemisia annua 9507
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-copalyl diphosphate Artemisia annua
-
 isopimara-7,15-diene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Artemisia annua A0A8B0RHM8 cv. Huhao 1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Artemisia annua
-
additional information ent-isopimara-7,15-diene synthase AaKSL1 is widely expressed in leaves, stems, and roots. Ent-isopimara-7,15-diene accumulates in both leaves and roots, metabolite analysis Artemisia annua
-
root
-
 Artemisia annua
-
stem
-
 Artemisia annua
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl diphosphate
-
 Artemisia annua isopimara-7,15-diene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
AaKSL1
-
 Artemisia annua
ent-isopimara-7,15-diene synthase
-
 Artemisia annua
KSL1
-
 Artemisia annua

Expression

Organism Comment Expression
Artemisia annua methyljasmonate induces AaKSL1 enzyme expression up

General Information

General Information Comment Organism
metabolism a complex repertoire of diterpenoids is found in Artemisia annua with important roles in glandular trichome (GT) formation, artemisinin accumulation and stress resilience. Metabolic engineering of diterpenoids simultaneously increases the artemisinin yield and stress resistance. Transcriptome and metabolic profiling suggests that bioactive gibberellins (GAs) GA4/GA1 promote GT formation. Protein-protein interactions (PPIs) between AaKSL1 and AaCPS2 in the plastids highlighted their potential functions in modulating metabolic flux to gibberellins (GAs) or ent-isopimara-7,15-diene-derived metabolites (IDMs) through metabolic engineering Artemisia annua
physiological function enzymes from diTPS class II and class I, AaCPS2 and AaKSL1, respectively, interact and function in specialized diterpenoid metabolism as a defense against stress. AaKSL1 converts ent-copalyl diphosphate (ent-CPP) into ent-isopimara-7,15-diene. Simultaneous overexpression of AaCPS2 and AaKSL1 may increase gibberellic acid and ent-isopimara-7,15-diene-derived metabolite (IDM) biosynthesis at the same time. Antifungal activity of ent-isopimara-7,15-diene-derived metabolites against Fusarium oxysporum Artemisia annua