Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl diphosphate | - |
Antillogorgia elisabethae | |
additional information | no inhibition by 0.05 mM N-ethylmaleimide | Antillogorgia elisabethae | |
oxo(phenyl)acetaldehyde | - |
Antillogorgia elisabethae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
geranylgeranyl diphosphate | 29°C | Antillogorgia elisabethae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the enzyme can not catalyze the cyclization reaction in the absence of Mg2+, saturating concentration is 1.5 mM, Km-value: 0.78 mM. At a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed | Antillogorgia elisabethae | |
Mn2+ | at a concentration of 2 mM, Mg2+ supports the cyclization reaction most efficiently. When substituting Mn2+ for Mg2+, approximately one-third of the elisabethatriene synthase activity is observed | Antillogorgia elisabethae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Antillogorgia elisabethae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Antillogorgia elisabethae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Antillogorgia elisabethae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
geranylgeranyl diphosphate | - |
Antillogorgia elisabethae | elisabethatriene + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, SDS-PAGE | Antillogorgia elisabethae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
highest activity in phosphate buffer | Antillogorgia elisabethae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Antillogorgia elisabethae | isoelectric focusing, pH-range 5-8 | - |
5.1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
4.5 | - |
29°C | Antillogorgia elisabethae | oxo(phenyl)acetaldehyde | |
4.9 | - |
29°C | Antillogorgia elisabethae | diethyl diphosphate |