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Literature summary for 4.2.3.4 extracted from
- Functional characterization by genetic complementation of aroB-encoded dehydroquinate synthase from Mycobacterium tuberculosis H37Rv and its heterologous expression and purification (2007), J. Bacteriol., 189, 6246-6252.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzymes of the shikimate pathway are attractive drug targets for the treatment of tuberculosis | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the pET23a+ vector for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Mycobacterium tuberculosis |  |
| Zn2+ | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38100 | - |
determined by SDS-PAGE | Mycobacterium tuberculosis |
| 38140 | - |
determined by ESI-MS | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | Mycobacterium tuberculosis | - |
3-dehydroquinate + phosphate | - |
ir | |
| 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | Mycobacterium tuberculosis H37Rv | - |
3-dehydroquinate + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WPX9 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WPX9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Q-Sepharose Fast Flow, a HiLoad 16/10 phenyl-Sepharose, and a Sephacryl S-200 HR column | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Mycobacterium tuberculosis | 3-dehydroquinate + phosphate | - |
ir | |
| 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate | - |
Mycobacterium tuberculosis H37Rv | 3-dehydroquinate + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 3-dehydroquinate synthase | - |
Mycobacterium tuberculosis |
| dehydroquinate synthase | - |
Mycobacterium tuberculosis |
| DHQ synthase | - |
Mycobacterium tuberculosis |
| DHQS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
activity assay | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
activity assay | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Mycobacterium tuberculosis | |
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Release 2023.1 (January 2023)
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