Cloned (Comment) | Organism |
---|---|
cloning from a cDNA library, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain XL1-Blue | Artemisia annua |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | highly activating at 0.060 mM, inhibiting at 0.12 mM | Artemisia annua |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
(2E,6E)-farnesyl diphosphate | pH 7.0, 25°C, recombinant enzyme | Artemisia annua | |
0.0013 | - |
(2E,6E)-farnesyl diphosphate | pH 9.0, 25°C, recombinant enzyme | Artemisia annua |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates at 2 mM | Artemisia annua | |
Mn2+ | highly activating at 0.060 mM, inhibiting at 0.12 mM | Artemisia annua |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63500 | - |
x * 63500, about, sequence calculation | Artemisia annua |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | Artemisia annua | the enzyme is involved in the artemisinin biosynthesis pathway, overview | 8-epi-cedrol + diphosphate | - |
? | |
additional information | Artemisia annua | Sesquiterpene cyclases or synthases catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Artemisia annua | Q9LLR9 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O = 8-epi-cedrol + diphosphate | reaction mechanism, overview | Artemisia annua |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Artemisia annua | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E,6E)-farnesyl diphosphate + H2O | the enzyme is involved in the artemisinin biosynthesis pathway, overview | Artemisia annua | 8-epi-cedrol + diphosphate | - |
? | |
(2E,6E)-farnesyl diphosphate + H2O | the native enzyme is specific for farnesyl diphosphate as substrate | Artemisia annua | 8-epi-cedrol + diphosphate | - |
? | |
geranyl diphosphate + H2O | geranyl diphosphate is converted to monoterpenes by the recombinant enzyme at a rate of about 15% of that observed with farnesyl diphosphate as substrate | Artemisia annua | ? + diphosphate | - |
? | |
additional information | Sesquiterpene cyclases or synthases catalyze the conversion of the isoprenoid intermediate farnesyl diphosphate to various sesquiterpene structural types | Artemisia annua | ? | - |
? | |
additional information | product formation specificity, overview. Native epi-cedrol synthase is not active with geranylgeranyl diphosphate as substrate. The recombinant enzyme catalyzes the formation of both olefinic sesquiterpene, i.e. 57% alpha-cedrene, 13% beta-cedrene, 5% (E)-beta-farnesene , 1% alpha-acoradiene, 8% (E)-alpha-bisabolene, and 16% of three unknown olefins, but mainly oxygenates sesquiterpenes, 97% of total sesquiterpene generated, composed of 96% epi-cedrol and 4% cedrol, from farnesyl diphosphate, GC-MS product analysis, overview | Artemisia annua | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63500, about, sequence calculation | Artemisia annua |
Synonyms | Comment | Organism |
---|---|---|
sesquiterpene cyclase | - |
Artemisia annua |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Artemisia annua |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | alcohol product formation | Artemisia annua |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.3 | 9 | - |
Artemisia annua |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Artemisia annua | sequence calculation | - |
4.94 |