Literature summary for 4.2.3.36 extracted from

Hamano, Y.; Kuzuyama, T.; Itoh, N.; Furihata, K.; Seto, H.; Dairi, T.
Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin (2002), J. Biol. Chem., 277, 37098-37104.

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Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	20% glycerol, 5 mM 2-mercaptoethanol, and 0.1% Tween 80 are required for the full activity of the Cy2c2	Kitasatospora griseola

Cloned(Commentary)

Cloned (Comment)	Organism
-	Kitasatospora griseola

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0076	-	terpentedienyl diphosphate	-	Kitasatospora griseola

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	1 mM, 3% of the activity with Mg2+, substrate: terpentedienyl diphosphate	Kitasatospora griseola
Fe2+	1 mM, 5% of the activity with Mg2+, substrate: terpentedienyl diphosphate	Kitasatospora griseola
Mg2+	required, substrate: terpentedienyl diphosphate	Kitasatospora griseola
Mn2+	1 mM, 37% of the activity with Mg2+, substrate: terpentedienyl diphosphate	Kitasatospora griseola

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37000	-	2 * 37000, calculated from sequence	Kitasatospora griseola
70000	-	gel filtration	Kitasatospora griseola

Organism

Organism	UniProt	Comment	Textmining
Kitasatospora griseola	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
terpentedienyl diphosphate	-	Kitasatospora griseola	terpentetriene + diphosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 37000, calculated from sequence	Kitasatospora griseola

Synonyms

Synonyms	Comment	Organism
Cyc2	-	Kitasatospora griseola

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	0.05 M Tris-HCl buffer	Kitasatospora griseola

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	-	stable after incubation at 30°C in 0.05 M Tris-HCl buffer (pH 6.8) for 1 h	Kitasatospora griseola

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.8	-	Tris-HCl buffer, substrate: terpentedienyl diphosphate	Kitasatospora griseola

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.8	7.5	-	Kitasatospora griseola

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Release 2023.1 (January 2023)