Application | Comment | Organism |
---|---|---|
synthesis | increase of levopimaradiene synthesis in Escherichia coli by amplification of the flux toward isopentenyl diphosphate and dimethylallyl diphosphate precursors and reprogramming the rate-limiting downstream pathway by generating combinatorial mutations in geranylgeranyl diphosphate synthase and levopimaradiene synthase. The most productive pathway, combining precursor flux amplification and mutant synthases, confers approximately 2600fold increase in levopimaradiene levels. A maximum titer of approximately 700 mg/l is obtained by cultivation in a benchscale bioreactor | Ginkgo biloba |
Protein Variants | Comment | Organism |
---|---|---|
A620T | 130% of wild-type productivity. Products are 87% levopimaradiene, 11% abieta-7,13-diene, and 2% sandaracopimaradiene | Ginkgo biloba |
A727S | inactive | Ginkgo biloba |
A729G | 2% of wild-type productivity. Product is 100% sandaracopimaradiene | Ginkgo biloba |
C618N | 20% of wild-type productivity. Products are 91% levopimaradiene, 9% abieta-7,13-diene, and traces of sandaracopimaradiene | Ginkgo biloba |
E777A | inactive | Ginkgo biloba |
G854T | 140% of wild-type productivity. Products are 79% levopimaradiene, 14% abieta-7,13-diene, and 7% sandaracopimaradiene | Ginkgo biloba |
I855L | 70% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene | Ginkgo biloba |
K723S | 180% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene | Ginkgo biloba |
L619F | 150% of wild-type productivity. Products are 94% levopimaradiene, 6% abieta-7,13-diene, and traces of sandaracopimaradiene | Ginkgo biloba |
L696Q | 160% of wild-type productivity. Products are 86% levopimaradiene, 7% abieta-7,13-diene, and 7% sandaracopimaradiene | Ginkgo biloba |
M593I | 370% of wild-type productivity. Products are 83% levopimaradiene, 12% abieta-7,13-diene, and 5% sandaracopimaradiene | Ginkgo biloba |
N769A | inactive | Ginkgo biloba |
N838E | 220% of wild-type productivity. Products are 92% levopimaradiene, 6% abieta-7,13-diene, and 2% sandaracopimaradiene | Ginkgo biloba |
V731L | 8% of wild-type productivity. Product is 100% levopimaradiene | Ginkgo biloba |
Y700F | 500% of wild-type productivity. Products are 80% levopimaradiene, 15% abieta-7,13-diene, and 5% sandaracopimaradiene | Ginkgo biloba |
Y700H | 40% of wild-type productivity. Products are 71% levopimaradiene, traces of abieta-7,13-diene, and 29% sandaracopimaradiene | Ginkgo biloba |
Y700M | 500% of wild-type productivity. Products are 80% levopimaradiene, 13% abieta-7,13-diene, and 7% sandaracopimaradiene | Ginkgo biloba |
Y700W | 400% of wild-type productivity. Products are 85% levopimaradiene, 7% abieta-7,13-diene, and 8% sandaracopimaradiene | Ginkgo biloba |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ginkgo biloba | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-copalyl diphosphate | - |
Ginkgo biloba | abieta-8(14),12-diene + diphosphate | products are levopimaradiene, abieta-7,13-diene, and sandaracopimaradiene | ? |