Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.2.3.32 extracted from

  • Leonard, E.; Ajikumar, P.K.; Thayer, K.; Xiao, W.H.; Mo, J.D.; Tidor, B.; Stephanopoulos, G.; Prather, K.L.
    Combining metabolic and protein engineering of a terpenoid biosynthetic pathway for overproduction and selectivity control (2010), Proc. Natl. Acad. Sci. USA, 107, 13654-13659.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis increase of levopimaradiene synthesis in Escherichia coli by amplification of the flux toward isopentenyl diphosphate and dimethylallyl diphosphate precursors and reprogramming the rate-limiting downstream pathway by generating combinatorial mutations in geranylgeranyl diphosphate synthase and levopimaradiene synthase. The most productive pathway, combining precursor flux amplification and mutant synthases, confers approximately 2600fold increase in levopimaradiene levels. A maximum titer of approximately 700 mg/l is obtained by cultivation in a benchscale bioreactor Ginkgo biloba

Protein Variants

Protein Variants Comment Organism
A620T 130% of wild-type productivity. Products are 87% levopimaradiene, 11% abieta-7,13-diene, and 2% sandaracopimaradiene Ginkgo biloba
A727S inactive Ginkgo biloba
A729G 2% of wild-type productivity. Product is 100% sandaracopimaradiene Ginkgo biloba
C618N 20% of wild-type productivity. Products are 91% levopimaradiene, 9% abieta-7,13-diene, and traces of sandaracopimaradiene Ginkgo biloba
E777A inactive Ginkgo biloba
G854T 140% of wild-type productivity. Products are 79% levopimaradiene, 14% abieta-7,13-diene, and 7% sandaracopimaradiene Ginkgo biloba
I855L 70% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene Ginkgo biloba
K723S 180% of wild-type productivity. Products are 90% levopimaradiene, 7% abieta-7,13-diene, and 3% sandaracopimaradiene Ginkgo biloba
L619F 150% of wild-type productivity. Products are 94% levopimaradiene, 6% abieta-7,13-diene, and traces of sandaracopimaradiene Ginkgo biloba
L696Q 160% of wild-type productivity. Products are 86% levopimaradiene, 7% abieta-7,13-diene, and 7% sandaracopimaradiene Ginkgo biloba
M593I 370% of wild-type productivity. Products are 83% levopimaradiene, 12% abieta-7,13-diene, and 5% sandaracopimaradiene Ginkgo biloba
N769A inactive Ginkgo biloba
N838E 220% of wild-type productivity. Products are 92% levopimaradiene, 6% abieta-7,13-diene, and 2% sandaracopimaradiene Ginkgo biloba
V731L 8% of wild-type productivity. Product is 100% levopimaradiene Ginkgo biloba
Y700F 500% of wild-type productivity. Products are 80% levopimaradiene, 15% abieta-7,13-diene, and 5% sandaracopimaradiene Ginkgo biloba
Y700H 40% of wild-type productivity. Products are 71% levopimaradiene, traces of abieta-7,13-diene, and 29% sandaracopimaradiene Ginkgo biloba
Y700M 500% of wild-type productivity. Products are 80% levopimaradiene, 13% abieta-7,13-diene, and 7% sandaracopimaradiene Ginkgo biloba
Y700W 400% of wild-type productivity. Products are 85% levopimaradiene, 7% abieta-7,13-diene, and 8% sandaracopimaradiene Ginkgo biloba

Organism

Organism UniProt Comment Textmining
Ginkgo biloba
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl diphosphate
-
Ginkgo biloba abieta-8(14),12-diene + diphosphate products are levopimaradiene, abieta-7,13-diene, and sandaracopimaradiene ?