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Literature summary for 4.2.3.32 extracted from

  • Schepmann, H.G.; Pang, J.; Matsuda, S.P.T.
    Cloning and characterization of Ginkgo biloba levopimaradiene synthase, which catalyzes the first committed step in ginkgolide biosynthesis (2001), Arch. Biochem. Biophys., 392, 263-269.
    View publication on PubMed
Search for more literature for 4.2.3.32

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol optimal activity in the presence of 5 mM DTT and 5% glycerol Ginkgo biloba

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Ginkgo biloba

Protein Variants

Protein Variants Comment Organism
additional information removal of 60 or 79 N-terminal residues increases levopimaradiene production, deletion of 128 N-terminal residues results in loss of catalytic activity Ginkgo biloba

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid sequence includes a putative N-terminal plastid transit peptide Ginkgo biloba 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Ginkgo biloba
additional information enzyme is not dependent on Mg2+ Ginkgo biloba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100289
-
 x * 100289, calculated Ginkgo biloba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ginkgo biloba catalyzes an intermediate step in synthesis of ginkgolide A ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ginkgo biloba Q947C4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information sequence includes a putative N-terminal plastid transit peptide and three aspartate-rich regions Ginkgo biloba

Source Tissue

Source Tissue Comment Organism Textmining
root root of seedling Ginkgo biloba
-
seedling root of seedling Ginkgo biloba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-copalyl-diphosphate reaction intermediate is labdadienyl diphosphate Ginkgo biloba levopimaradiene + diphosphate
-
 ?
additional information catalyzes an intermediate step in synthesis of ginkgolide A Ginkgo biloba ?
-
 ?

Subunits

Subunits Comment Organism
? x * 100289, calculated Ginkgo biloba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Ginkgo biloba