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Literature summary for 4.2.3.31 extracted from

  • Ikeda, C.; Hayashi, Y.; Itoh, N.; Seto, H.; Dairi, T.
    Functional analysis of eubacterial ent-copalyl diphosphate synthase and pimara-9(11),15-diene synthase with unique primary sequences (2007), J. Biochem., 141, 37-45.
    View publication on PubMed
Search for more literature for 4.2.3.31

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 5 mM, required for full activity Streptomyces sp.
Tween-80 0.1%, required for full activity Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00026
-
 ent-copalyl diphosphate 30°C, pH 7.0 Streptomyces sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 1 mM, 100% of the activity with Mg2+ Streptomyces sp.
Mg2+ absolutely required, optimal concentraion 1 mM Streptomyces sp.
additional information no or very weak activity in presence of Mn2+, Ca2+, Cu2+ and Fe2+ Streptomyces sp.
Ni2+ 1 mM, 57% of the activity with Mg2+ Streptomyces sp.
Zn2+ 1 mM, 59% of the activity with Mg2+ Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Streptomyces sp.

Organism

Organism UniProt Comment Textmining
Streptomyces sp.
-
-
-
Streptomyces sp. KO-3988
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ent-copalyl diphosphate
-
 Streptomyces sp. pimara-9(11),15-diene + diphosphate
-
 ?
ent-copalyl diphosphate
-
 Streptomyces sp. KO-3988 pimara-9(11),15-diene + diphosphate
-
 ?
additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate Streptomyces sp. ?
-
 ?
additional information no substrates: farnesyl diphosphate, geranylgeranyl diphosphate, terpentecin diphosphate Streptomyces sp. KO-3988 ?
-
 ?

Subunits

Subunits Comment Organism
monomer x * 35000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 0.05 M Tris, pH 7.0 Streptomyces sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 5.5, stable for at least 1 h Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
 ent-copalyl diphosphate 30°C, pH 7.0 Streptomyces sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptomyces sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Streptomyces sp.