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Literature summary for 4.2.3.3 extracted from

  • Zareian, S.; Khajeh, K.; Pazhang, M.; Ranjbar, B.
    Rationalization of allosteric pathway in Thermus sp. GH5 methylglyoxal synthase (2012), BMB Rep., 45, 748-753.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.3.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Thermus sp.

Protein Variants

Protein Variants Comment Organism
D10N almost complete loss of activity Thermus sp.
additional information mutant enzyme with C-terminal extension YQRYLADRLK to arrange a salt bridge between Arg140 and the proximal aspartate at position 10. Mutant shows an increase in Hill coefficient from 1.5 of wild-type to 1.99 and an increase in thermostability. Introduction of mutation D10N in this mutant reverts the Hill coefficient to 1.44 Thermus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
0.25
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
0.5
-
 dihydroxyacetone phosphate wild-type, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
0.75
-
 dihydroxyacetone phosphate mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.

Organism

Organism UniProt Comment Textmining
Thermus sp. B3VH91
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate
-
 Thermus sp. methylglyoxal + phosphate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 wild-type, half-life 3 min, mutant with C-terminal extension YQRYLADRLK, half-life 10 min Thermus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
0.41
-
 dihydroxyacetone phosphate mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
103
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
326
-
 dihydroxyacetone phosphate wild-type, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.374
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK C-terminal extension YQRYLADRLK plus mutation D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
0.544
-
 dihydroxyacetone phosphate mutant D10N, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
490
-
 dihydroxyacetone phosphate mutant carrying C-terminal extension YQRYLADRLK, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.
653
-
 dihydroxyacetone phosphate wild-type, pH 6.0, 60°C, Hill coefficient 1.5 Thermus sp.