Literature summary for 4.2.3.3 extracted from

Pazhang, M.; Khajeh, K.; Asghari, S.M.; Falahati, H.; Naderi-Manesh, H.
Cloning, expression, and characterization of a novel methylglyoxal synthase from Thermus sp. strain GH5 (2010), Appl. Biochem. Biotechnol., 162, 1519-1528.

Search for more literature for 4.2.3.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)	Thermus sp. GH5

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.56	-	glycerone phosphate	allosteric enzyme	Thermus sp. GH5

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14300	-	6 * 14300, gel filtration, SDS-PAGE	Thermus sp. GH5
89000	-	gel filtration	Thermus sp. GH5

Organism

Organism	UniProt	Comment	Textmining
Thermus sp. GH5	B3VH91	-	-

Purification (Commentary)

Purification (Comment)	Organism
heat shock (70°C, 15 min), anion-exchange chromatography (Q-Sepharose)	Thermus sp. GH5

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6000	-	-	Thermus sp. GH5

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycerone phosphate	-	Thermus sp. GH5	pyruvaldehyde + phosphate	tautomerization to methylglyoxal	?

Subunits

Subunits	Comment	Organism
homohexamer	6 * 14300, gel filtration, SDS-PAGE	Thermus sp. GH5

Synonyms

Synonyms	Comment	Organism
methylglyoxal synthase	-	Thermus sp. GH5

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
75	-	-	Thermus sp. GH5

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-	75	100% activity after 15 min	Thermus sp. GH5

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
325	-	glycerone phosphate	-	Thermus sp. GH5

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Thermus sp. GH5

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Release 2023.1 (January 2023)