Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D101E | reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme | Escherichia coli |
D101N | reduced ratio of turnover-numer:Km-value by about 10000fold compared to the wild-type enzyme | Escherichia coli |
D71E | reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme | Escherichia coli |
D71N | reduced ratio of turnover-numer:Km-value by about 1000fold compared to the wild-type enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-phosphoglycerate | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
dihydroxyacetone phosphate | wild-type enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
4 * 17000, SDS-PAGE | Escherichia coli |
67000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type enzyme and mutant enzymes D71E, D71N, D101E and D101N | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate | - |
Escherichia coli | methylglyoxal + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 17000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
220 | - |
dihydroxyacetone phosphate | wild-type enzyme | Escherichia coli |