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Literature summary for 4.2.3.27 extracted from
- Biochemical characterization of isoprene synthase from Ipomoea batatas (2019), J. Biosci. Bioeng., 127, 138-144 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Ipomoea batatas |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | no activity in the presence of Ca2+ | Ipomoea batatas |  |
| EDTA | no activity in the presence of EDTA | Ipomoea batatas | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
dimethylallyl diphosphate | at pH 7.8 and 37°C | Ipomoea batatas | |
| 0.34 | - |
dimethylallyl diphosphate | at pH 7.8 and 37°C | Populus alba | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ with optimum concentration of 56 mM is needed for enzyme activation | Ipomoea batatas | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | Populus alba | - |
isoprene + diphosphate | - |
? | |
| dimethylallyl diphosphate | Ipomoea batatas | - |
isoprene + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ipomoea batatas | - |
- |
- |
| Populus alba | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| BeyoGold His-tag purification resin column chromatography | Ipomoea batatas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate | - |
Populus alba | isoprene + diphosphate | - |
? | |
| dimethylallyl diphosphate | - |
Ipomoea batatas | isoprene + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Ipomoea batatas |
| ? | x * 65000, SDS-PAGE | Populus alba |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ISPS | - |
Populus alba |
| ISPS | - |
Ipomoea batatas |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Ipomoea batatas |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | after incubation at 30°C for 10 min, 62% of the enzyme activity remains. 8% of the enzyme activity remains after 60 min of incubation at 30°C. After 10 min at 40°C, most of the enzyme activity (84%) is lost. Almost no enzyme activity remains after 40 min at 40°C. When the enzyme is incubated at 50°C or 60°C, no enzyme activity is detected after 10 min | Ipomoea batatas |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
dimethylallyl diphosphate | at pH 7.8 and 37°C | Populus alba | |
| 0.37 | - |
dimethylallyl diphosphate | at pH 7.8 and 37°C | Ipomoea batatas | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Ipomoea batatas |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
89% enzyme activity is lost after 20 min of incubation at pH 7.0 | Ipomoea batatas |
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