KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0038 | - |
dimethylallyl diphosphate | Km of 3.8 miromol/qm are estimated from the Lineweaver-Burks plot | Populus tremula x Populus tremuloides | |
0.26 | - |
dimethylallyl diphosphate | using an appropriate conversion factor between area-and volume-based units | Populus tremula x Populus tremuloides |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Populus tremula x Populus tremuloides | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Populus tremula x Populus tremuloides | the control on foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyl diphosphate and the pool size of dimethyl allyldiphosphate | isoprene + diphosphate | - |
? | |
additional information | Populus tremula x Populus tremuloides | dimethylallyl diphosphate pool is directly associated with isoprene synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremula x Populus tremuloides | - |
hybrid | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | from one year old seedlings of hybrid aspen clone 200 which are grown under photosynthetic photon flux density of 0.5 mmol qm/s with 14 light period. Air temperature is kept at 25°C during the day and 20°C during the night, relative air humidity is 60%. Plants are watered daily with distilled water and once per weak with a combined nutrient solution containing macro- and microelements | Populus tremula x Populus tremuloides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of isoprene synthase remains constant during postillumination process | Populus tremula x Populus tremuloides |
additional information | - |
isoprene synthase mainly operates under substrate limitation and short-term light, CO2 and O2 dependencies of isoprene emission result from variation in dimethylallyl diphosphate pool size rather than from modifications in isoprene synthase activity | Populus tremula x Populus tremuloides |
additional information | - |
maximum capacity of isoprene synthase of Vmax of 110 nmol/qm/s are estimated | Populus tremula x Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | the control on foliar isoprene emission is shared between the activity of isoprene synthase, the terminal enzyme catalyzing isoprene formation from dimethylallyl diphosphate and the pool size of dimethyl allyldiphosphate | Populus tremula x Populus tremuloides | isoprene + diphosphate | - |
? | |
additional information | dimethylallyl diphosphate pool is directly associated with isoprene synthesis | Populus tremula x Populus tremuloides | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
isoprene synthase | - |
Populus tremula x Populus tremuloides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.7 | 8.2 | broad pH-optimum | Populus tremula x Populus tremuloides |