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Literature summary for 4.2.3.27 extracted from

  • Sasaki, K.; Saito, T.; Laemsae, M.; Oksman-Caldentey, K.M.; Suzuki, M.; Ohyama, K.; Muranaka, T.; Ohara, K.; Yazaki, K.
    Plants utilize isoprene emission as a thermotolerance mechanism (2007), Plant Cell Physiol., 48, 1254-1262.
    View publication on PubMed
Search for more literature for 4.2.3.27

Cloned(Commentary)

Cloned (Comment) Organism
the plasmid pGWB2-PaIspS is introduced via Agrobacterium tumefaciens into Arabidopsis plants Populus alba

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
 Populus alba 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Populus alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate Populus alba
-
 isoprene + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Populus alba Q50L36
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Populus alba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1500
-
 cell free exract from leaf transformants Populus alba
4967
-
 native level in Populus alba leaves Populus alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate
-
 Populus alba isoprene + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
isoprene synthase
-
 Populus alba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 activity assay, volatile compounds of the reaction are trapped with Solid-Phase Micro Extraction fiber, which is directly injected to a gas-liqiud chromatograph Populus alba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Populus alba