Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is positively regulated by light | Populus tremuloides |
General Stability | Organism |
---|---|
glycerol stabilizes the enzyme in the early stages of purification, PEG in the late stages | Populus tremuloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dimethylallyl diphosphate | substrate inhibition above 10 mM | Populus tremuloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8 | - |
dimethylallyl diphosphate | - |
Populus tremuloides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Populus tremuloides | |
Mn2+ | can substitute for Mg2+ | Populus tremuloides | |
additional information | the enzyme is strictly dependent on divalent cations, no activity with Zn2+ and Ca2+ | Populus tremuloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
63000 | - |
1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
98000 | 137000 | gel filtration | Populus tremuloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | Populus tremuloides | - |
isoprene + diphosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus tremuloides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 4000fold from leaves to homogeneity, ammonium sulfate fractionation of cell-free supernatant, dialysis, ion exchange chromatography, ultrafiltration, and gel filtration | Populus tremuloides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Populus tremuloides | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.521 | - |
purified enzyme | Populus tremuloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | - |
ir | |
dimethylallyl diphosphate | - |
Populus tremuloides | isoprene + diphosphate | product stoichiometry determination, isoprene is volatile 2-methyl-1,3-butadiene emitted by the plant into the atmosphere, where it plays an important role, the enzyme is positively regulated by light | ir |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 58000 + 1 * 63000, SDS-PAGE | Populus tremuloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
assay at | Populus tremuloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
dimethylallyl diphosphate | - |
Populus tremuloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Populus tremuloides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Populus tremuloides | isoelectric focusing | - |
4.7 |